Virus-based photo-responsive nanowires formed by linking site-directed mutagenesis and chemical reaction.
Virus-based photo-responsive nanowires formed by linking site-directed mutagenesis and chemical reaction.
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DOI：
10.1038/srep01820
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发表时间：
2013
影响因子：
4.6
通讯作者：
Mao C
中科院分区：
文献类型：
--
作者：
Murugesan M;Abbineni G;Nimmo SL;Cao B;Mao C
生物纳米结构（如丝状噬菌体）由于其遗传的灵活性和无差错的批量生产，在材料合成方面显示出巨大的潜力，但其光响应行为尚未被探索或揭示。在这里，我们展示了M13噬菌体基因工程与酪氨酸残基精确融合的主要外壳蛋白转化成光响应的有机纳米线通过与芳香胺的位点特异性化学反应在表面上形成偶氮染料结构。由于在噬菌体上形成的偶氮单元的光可切换的顺反异构化，所得到的偶氮-M13-噬菌体纳米线表现出可逆的光响应性质。这一结果表明，通过定点遗传诱变在生物纳米结构上位点特异性展示肽可以转化为用于开发先进材料的定点化学反应。偶氮-M13-噬菌体纳米线的光响应特性可能为开发用于非线性光学、全息数据存储、分子天线和致动器的光可控智能器件打开大门。
Owing to the genetic flexibility and error-free bulk production, bio-nanostructures such as filamentous phage showed great potential in materials synthesis, however, their photo-responsive behaviour is neither explored nor unveiled. Here we show M13 phage genetically engineered with tyrosine residues precisely fused to the major coat protein is converted into a photo-responsive organic nanowire by a site-specific chemical reaction with an aromatic amine to form an azo dye structure on the surface. The resulting azo-M13-phage nanowire exhibits reversible photo-responsive properties due to the photo-switchable cis-trans isomerisation of the azo unit formed on the phage. This result shows that site-specific display of a peptide on bio-nanostructures through site-directed genetic mutagenesis can be translated into site-directed chemical reaction for developing advanced materials. The photo-responsive properties of the azo-M13-phage nanowires may open the door for the development of light controllable smart devices for use in non-linear optics, holography data storage, molecular antenna, and actuators.
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影响因子：
2.5
作者：
Cao, Binrui;Xu, Hong;Mao, Chuanbin
通讯作者：
Mao, Chuanbin
DOI：
10.1073/pnas.0403795101
发表时间：
2004-07-13
影响因子：
11.1
作者：
Carrera, MRA;Kaufmann, GF;Janda, KD
通讯作者：
Janda, KD
影响因子：
1.8
作者：
Ghosh, S;Banthia, AK
通讯作者：
Banthia, AK
影响因子：
11.2
作者：
Krag, David N.;Shukla, Girja S.;Thomas, Christian
通讯作者：
Thomas, Christian
影响因子：
3.6
作者：
Bandara, H. M. Dhammika;Friss, Tracey R.;Burdette, Shawn C.
通讯作者：
Burdette, Shawn C.