Transmission electron microscopy as a tool to image bioinorganic nanohybrids: the case of phage-gold nanocomposites.
Transmission electron microscopy as a tool to image bioinorganic nanohybrids: the case of phage-gold nanocomposites.
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DOI：
10.1002/jemt.21030
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发表时间：
2011-07
影响因子：
2.5
通讯作者：
Mao, Chuanbin
中科院分区：
文献类型：
--
作者：
Cao, Binrui;Xu, Hong;Mao, Chuanbin
近年来，由生物大分子和功能性无机纳米材料组成的生物无机纳米杂化材料展现出许多独特的性能，展现出广阔的前景。透射电子显微镜 (TEM) 是一种流行且相对简单的工具，可以以高分辨率直接观察纳米材料。当应用 TEM 可视化生物无机纳米杂化物时，由于纳米杂化物中存在生物分子（除了那些具有密集堆积的无机材料的纳米杂化物），需要进行负染色处理。然而，常规生物样品的常规负染色程序不能直接应用于此类生物无机纳米杂化物。为了对特定的生物无机纳米杂化物进行成像，应确定负染色因素，例如负染色类型、工作 pH 值、染色时间和干燥方法。目前，还没有详细的研究来研究如何基于特定的生物无机纳米杂化物来调整负染色因子。本研究选择噬菌体-金纳米颗粒杂化物作为模型，系统研究各因素对纳米杂化物负染色的影响。获得了金纳米粒子-噬菌体纳米杂交体的最佳染色条件，并讨论了各因素对一般纳米杂交体阴性染色的影响。这项工作表明，通过适当的染色，可以使用 TEM 直接可视化生物和无机成分，而不会引入任何伪影。
In recent years, bio-inorganic nanohybrids composed of biological macromolecules and functional inorganic nanomaterials have revealed many unique properties that show promise for the future. Transmission electron microscopy (TEM) is a popular and relatively simple tool that can offer a direct visualization of the nanomaterials with high resolutions. When TEM is applied to visualize bio-inorganic nanohybrids, a treatment of negative staining is necessary due to the presence of biological molecules in the nanohybrids except for those with densely packed inorganic materials. However, the conventional negative-staining procedure for regular biological samples cannot be directly applied to such bio-inorganic nanohybrids. To image a specific bio-inorganic nanohybrid, negative-staining factors such as negative stain type, working pH, staining time, and drying method, should be identified. Currently, no detailed studies have been done to investigate how to adjust negative-staining factors based on specific bio-inorganic nanohybrids. In this study, bacteriophage-gold nanoparticle hybrids were chosen as a model to systematically study the effects of each factor on the negative staining of the nanohybrids. The best staining conditions for gold nanoparticle-phage nanohybrids were obtained and the effects of each factor on the negative staining of general nanohybrids were discussed. This work indicates that with proper staining it is possible to use TEM to directly visualize both biological and inorganic components without introducing any artifact.
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影响因子：
10.8
作者：
Lin C;Ke Y;Li Z;Wang JH;Liu Y;Yan H
通讯作者：
Yan H
影响因子：
3.9
作者：
Cao, Binrui;Mao, Chuanbin
通讯作者：
Mao, Chuanbin
影响因子：
3
作者：
Pan, H.-B.;Darvell, B. W.
通讯作者：
Darvell, B. W.
DOI：
10.1126/science.1165831
发表时间：
2009-01-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Sharma J;Chhabra R;Cheng A;Brownell J;Liu Y;Yan H
通讯作者：
Yan H
影响因子：
3.8
作者：
Chen Zhuofan;Huang Baoxin;Darvell, Brian W.
通讯作者：
Darvell, Brian W.