Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells
Screening bioactive compounds from Ligusticum chuanxiong by high density immobilized human umbilical vein endothelial cells
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高密度固定化人脐静脉内皮细胞筛选川芎生物活性成分
DOI：
10.1007/s00216-015-8764-5
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发表时间：
2015-05
影响因子：
4.3
通讯作者：
Zheng, Xiaohui
中科院分区：
文献类型：
--
作者：
Sun, Huanmei;Bian, Liujiao;Zhao, Xinfeng;Zheng, Xiaohui
高通量筛选方法在从复杂介质中筛选生物活性化合物方面起着非常重要的作用。在这项工作中，一种以人脐静脉内皮细胞（HUVECs）为材料将细胞附着在氨基微球上的新策略……（原句最后“as a pro”不完整）
High throughput screening methodologies play a very important role in screening bioactive compounds from complex media. In this work, a new strategy for attaching cells onto amino microspheres using human umbilical vein endothelial cells (HUVECs) as a probe was developed. The immobilization depended on the specific affinity between integrin on the cells and the RGD peptide, which was coated on poly[oligo (ethylene glycol) methacrylate] by atom transfer radical polymerization. Validated application of the stationary phase was performed in the analysis of Ligusticum chuanxiong extraction by high performance affinity chromatography-mass spectrometry. Three compounds were screened as the bioactive compounds of Ligusticum chuanxiong. Two of them were identified as 3-butyl-hexahydroisobenzofuran-1(3H)-one and tetramethylpyrazine (TMP), whereas the other one remains indistinct. The association constant of vascular endothelial growth factor (VEGF) and TMP binding to VEGF receptor (VEGFR) on HUVECs were calculated to be (1.04 ± 0.08) × 10(11) M(-1) and (9.84 ± 1.11) × 10(8) M(-1) by zonal elution. Molecular docking showed that one hydrogen bond was formed between N atom of TMP and 3-N atom of imidazole group in histidine(223) of VEGFR. Both zonal elution and molecular docking indicated that TMP and VEGF bind to the same site of VEGFR on HUVECs. It is possible to become a promising tool for high throughput screening of the bioactive compounds binding to HUVECs through broad application of the stationary phase.
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影响因子：
14
作者：
Tugulu, Stefano;Silacci, Paolo;Klok, Harm-Anton
通讯作者：
Klok, Harm-Anton
影响因子：
3.4
作者：
P. Jeffrey
通讯作者：
P. Jeffrey
影响因子：
12.6
作者：
von Tiedemann, B;Bilitewski, U
通讯作者：
Bilitewski, U
影响因子：
1.7
作者：
T. Taguchi;A. Kishida;M. Akashi;I. Maruyama
通讯作者：
T. Taguchi;A. Kishida;M. Akashi;I. Maruyama
DOI：
10.1016/j.jchromb.2009.02.031
发表时间：
2009-04
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
Xinfeng Zhao;Xiaohui Zheng;Yinmao Wei;Liujiao Bian;Shixiang Wang;Jianbin Zheng;You-yi Zhang;Zi-jian Li;W. Zang
通讯作者：
Xinfeng Zhao;Xiaohui Zheng;Yinmao Wei;Liujiao Bian;Shixiang Wang;Jianbin Zheng;You-yi Zhang;Zi-jian Li;W. Zang