Collagen-induced arthritis as an animal model of rheumatoid cachexia.
Collagen-induced arthritis as an animal model of rheumatoid cachexia.
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DOI：
10.1002/jcsm.12280
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Xavier RM
中科院分区：
文献类型：
--
作者：
Alabarse PVG;Lora PS;Silva JMS;Santo RCE;Freitas EC;de Oliveira MS;Almeida AS;Immig M;Teixeira VON;Filippin LI;Xavier RM
类风湿性关节炎的特征是慢性多关节滑膜炎，并呈现影响生活质量的系统性变化，如肌肉功能受损，高达66%的患者可见。这会发展到被称为类风湿性恶病质的严重虚弱状态--没有脂肪质量和体重的损失--对于这种情况，在诊断或治疗方面几乎没有达成共识。本研究旨在评估胶原诱导性关节炎(CIA)动物模型是否也表现出类风湿性恶病质的临床和功能特征。雄性DBA1/J小鼠随机分为2组：健康组(CO，n=11)和CIA组(n=13)。分别于发病后0d、18d、35d、45d、55d、65d进行临床评分、水肿大小、动物体重和摄食量、自由探索运动、握力和耐力运动能力测试。安乐死后，对几个器官，内脏脂肪和棕色脂肪，以及肌肉进行解剖和称重。用肌肉来评估肌纤维直径。踝关节通过组织学评分评估关节炎的严重程度。采用单因素方差分析和双因素方差分析进行统计学分析，然后进行Tukey‘s和Bonferroni’s检验或Pearson‘s t检验，假设P值在0.05以下为统计学差异。CIA组的关节炎评分和后爪水肿量显著高于CO组。与CO相比，CIA的耐力运动表现总时间(疲劳；35、45、55和65天分别为23、22、24和21%)、握力(25、35、45、55和65天分别为27、55、63、60和66%)、自由活动(35、45、55和65天分别为43、57、59和66%)、胫骨前肌和腓肠肌重量(分别为25和24%)与CO相比降低。CIA组的肌浆比也降低(TA：23，GA：比CO低22%)，证实了这些动物的骨骼肌块比CO组萎缩。与CO组相比，TA组和GA组的肌纤维直径分别减小了45%和41%。CIA组内脏脂肪和棕色脂肪较CO组轻(分别为54%和39%)。CIA模型是一个有效的类风湿性恶病质实验模型，因为观察到的临床变化与类风湿性关节炎患者所描述的相似。
Rheumatoid arthritis is characterized by chronic polyarticular synovitis and presents systemic changes that impact quality of life, such as impaired muscle function, seen in up to 66% of the patients. This can progress to severely debilitating state known as rheumatoid cachexia—without loss of fat mass and body weight—for which there is little consensus in terms of diagnosis or treatment. This study aims to evaluate whether the collagen‐induced arthritis (CIA) animal model also develops clinical and functional features characteristic of rheumatoid cachexia. Male DBA1/J mice were randomly divided into 2 groups: healthy animals (CO, n = 11) and CIA animals (n = 13). The clinical score and edema size, animal weight and food intake, free exploratory locomotion, grip strength, and endurance exercise performance were tested 0, 18, 35, 45, 55, and 65 days after disease induction. After euthanasia, several organs, visceral and brown fat, and muscles were dissected and weighed. Muscles were used to assess myofiber diameter. Ankle joint was used to assess arthritis severity by histological score. Statistical analysis were performed using one‐way and two‐way analyses of variance followed by Tukey's and Bonferroni's test or t‐test of Pearson and statistical difference were assumed for a P value under 0.05. The CIA had significantly higher arthritis scores and larger hind paw edema volumes than CO. The CIA had decreased endurance exercise performance total time (fatigue; 23, 22, 24, and 21% at 35, 45, 55, and 65 days, respectively), grip strength (27, 55, 63, 60, and 66% at 25, 35, 45, 55, and 65 days, respectively), free locomotion (43, 57, 59, and 66% at 35, 45, 55, and 65 days, respectively), and tibialis anterior and gastrocnemius muscle weight (25 and 24%, respectively) compared with CO. Sarcoplasmic ratios were also reduced in CIA (TA: 23 and GA: 22% less sarcoplasmic ratio), confirming the atrophy of skeletal muscle mass in these animals than in CO. Myofiber diameter was also reduced 45% in TA and 41% in GA in CIA when compared with the CO. Visceral and brown fat were lighter in CIA (54 and 39%, respectively) than CO group. The CIA model is a valid experimental model for rheumatoid cachexia given that the clinical changes observed were similar to those described in patients with rheumatoid arthritis.
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影响因子：
4.7
作者：
Cooney JK;Law RJ;Matschke V;Lemmey AB;Moore JP;Ahmad Y;Jones JG;Maddison P;Thom JM
通讯作者：
Thom JM
补肺健脾颗粒改善慢性阻塞性肺疾病大鼠骨骼肌和线粒体功能障碍
影响因子：
--
作者：
Dong Y;Li Y;Sun Y;Mao J;Yao F;Tian Y;Wang L;Li L;Li S;Li J
通讯作者：
Li J
影响因子：
--
作者：
Oliveira, Silvia Cristina Gutierrez;Oliveira, Leda Magalhaes;Natour, Jamil
通讯作者：
Natour, Jamil
影响因子：
2.1
作者：
Engvall, I-L;Elkan, A-C;Hafstrom, I.
通讯作者：
Hafstrom, I.
影响因子：
--
作者：
Brenner, M;Meng, HC;Gulko, NS
通讯作者：
Gulko, NS