Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis
Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis
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金黄色葡萄球菌和表皮葡萄球菌种内和种间庆大霉素抗性质粒的接合转移
DOI：
10.1128/aac.23.1.151
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发表时间：
1983
影响因子：
4.9
通讯作者：
Sidney Coheni
中科院分区：
文献类型：
--
作者：
Richard W. McDonnell;H. Sweeney;Sidney Coheni
我们先前已经报道了在Michael Reese医院分离的金黄色葡萄球菌和表皮葡萄球菌菌株之间的种间和种内混合培养物中庆大霉素抗性（Gmr）质粒的转移（Jaffe等人，抗菌剂探员Chemother 21：773-779，1982）。我们现在已经证明，这些质粒的代表在明显非溶原性的链球菌菌株之间转移。金黄色葡萄球菌中，在肉汤中的混合培养物中或通过在琼脂培养基上过滤交配。转移的机制似乎是共轭。可转移的Gmr质粒（pSH 8）动员或共转移了不可独立转移的四环素R-质粒和氯霉素R-质粒。Gmr质粒的转移伴随着高发生率的缺失突变，具有不同的质粒抗性决定簇的损失，并且对于某些突变体，丧失了影响自身转移的能力。限制性内切酶消化的pSH 8和它的缺失突变体，使它有可能分配到一个特定的部分的pSH 8基因组的自我转移的属性，并提供了该质粒的物理和遗传图谱的基础。类似的Gmr质粒来自S.在远离Michael Reese医院的地方分离的金黄色葡萄球菌菌株具有与pSH 8相当的抗性决定簇和转移特性。我们的观察提供了证据，一些葡萄球菌质粒的接合转移，显然独立的噬菌体的存在。这种机制可能对葡萄球菌对氨基糖苷类和其他抗生素的耐药性的种内和种间传播具有重要意义。
We have previously reported the transfer of gentamicin resistance (Gmr) plasmids in a mixed culture inter- and intraspecifically between strains of Staphylococcus aureus and Staphylococcus epidermidis isolated at Michael Reese Hospital (Jaffe et al., Antimicrob. Agents Chemother. 21:773-779, 1982). We have now shown that representatives of these plasmids were transferred between apparently nonlysogenic strains of S. aureus either in mixed culture in broth or by filter-mating on agar medium. The mechanism of transfer appeared to be conjugation. A transferable Gmr plasmid (pSH8) mobilized or cotransferred a tetracycline R-plasmid and a chloramphenicol R-plasmid that were not independently transferable. The transfer of Gmr plasmids was accompanied by a high incidence of deletion mutations with varied loss of plasmid resistance determinants and, with some mutants, loss of the ability to effect self-transfer. Restriction endonuclease digestion of pSH8 and its deletion mutants made it possible to assign the property of self-transfer to a specific segment of the pSH8 genome and provided the basis for a physical and genetic map of that plasmid. Similar Gmr plasmids from S. aureus strains isolated in locations remote from Michael Reese Hospital had resistance determinants and transfer properties comparable to those of pSH8. Our observations provide evidence for the conjugal transfer of some staphylococcal plasmids, apparently independent of the presence of phage. This mechanism may be of significance in the intra- and interspecific dissemination of resistance to aminoglycosides and other antibiotics in Staphylococcus spp.
链球菌属的质粒、耐药性和基因转移。
DOI：
10.1128/mr.45.3.409-436.1981
发表时间：
1981
期刊：
Microbiological reviews
影响因子：
--
作者：
Clewell,DB
通讯作者：
Clewell,DB