piRNA-directed cleavage of meiotic transcripts regulates spermatogenesis.
piRNA-directed cleavage of meiotic transcripts regulates spermatogenesis.
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DOI：
10.1101/gad.260455.115
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发表时间：
2015-05-15
影响因子：
10.5
通讯作者：
Hannon GJ
中科院分区：
文献类型：
--
作者：
Goh WS;Falciatori I;Tam OH;Burgess R;Meikar O;Kotaja N;Hammell M;Hannon GJ
MIWI催化活性是精子发生所必需的，表明piRNA引导的切割对于生殖细胞发育至关重要。通过鉴定减数分裂皮尔纳靶点，这项研究支持了这样的观点，即减数分裂皮尔纳群体必须被强烈选择以实现成功的精子发生，既驱使反应远离必需基因，又将途径导向减数分裂细胞中由小RNA调节的mRNA靶点。MIWI催化活性是精子发生所必需的，表明piRNA引导的切割对于生殖细胞发育至关重要。为了鉴定减数分裂皮尔纳靶标，我们通过引入人减数分裂皮尔纳簇来增强小鼠皮尔纳库。这通过将皮尔纳机制不适当地靶向生殖细胞发育所必需的小鼠mRNA而引发精子发生缺陷。这种从头靶的分析揭示了粗线期皮尔纳靶识别的特征。这使得能够鉴定转座因子和减数分裂表达的蛋白质编码基因作为天然piRNA的靶标。基因靶的切割开始于粗线期阶段，并导致通过减数分裂进行性阻遏，至少部分通过乒乓循环驱动。我们的数据支持这样的观点，即减数分裂皮尔纳群体必须被强烈选择以实现成功的精子发生，既驱动反应远离必需基因，又将通路导向减数分裂细胞中由小RNA调节的mRNA靶点。
MIWI catalytic activity is required for spermatogenesis, indicating that piRNA-guided cleavage is critical for germ cell development. Through identifying meiotic piRNA targets, this study supports the idea that meiotic piRNA populations must be strongly selected to enable successful spermatogenesis, both driving the response away from essential genes and directing the pathway toward mRNA targets that are regulated by small RNAs in meiotic cells. MIWI catalytic activity is required for spermatogenesis, indicating that piRNA-guided cleavage is critical for germ cell development. To identify meiotic piRNA targets, we augmented the mouse piRNA repertoire by introducing a human meiotic piRNA cluster. This triggered a spermatogenesis defect by inappropriately targeting the piRNA machinery to mouse mRNAs essential for germ cell development. Analysis of such de novo targets revealed a signature for pachytene piRNA target recognition. This enabled identification of both transposable elements and meiotically expressed protein-coding genes as targets of native piRNAs. Cleavage of genic targets began at the pachytene stage and resulted in progressive repression through meiosis, driven at least in part via the ping-pong cycle. Our data support the idea that meiotic piRNA populations must be strongly selected to enable successful spermatogenesis, both driving the response away from essential genes and directing the pathway toward mRNA targets that are regulated by small RNAs in meiotic cells.
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DOI：
10.1261/rna.045310.114
发表时间：
2014-08
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Hirano T;Iwasaki YW;Lin ZY;Imamura M;Seki NM;Sasaki E;Saito K;Okano H;Siomi MC;Siomi H
通讯作者：
Siomi H
影响因子：
4
作者：
Escoffier, Jessica;Yassine, Sandra;Arnoult, Christophe
通讯作者：
Arnoult, Christophe
影响因子：
44.1
作者：
Beyret, Ergin;Liu, Na;Lin, Haifan
通讯作者：
Lin, Haifan
影响因子：
64.8
作者：
Reuter, Michael;Berninger, Philipp;Pillai, Ramesh S.
通讯作者：
Pillai, Ramesh S.
影响因子：
11.4
作者：
Castaneda, Julio;Genzor, Pavol;Bortvin, Alex
通讯作者：
Bortvin, Alex