Can too many copies spoil the broth?
Can too many copies spoil the broth?
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DOI：
10.1186/1475-2859-12-128
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发表时间：
2013-12-20
影响因子：
6.4
通讯作者：
Polizzi KM
中科院分区：
文献类型：
--
作者：
Aw R;Polizzi KM
巴斯德毕赤酵母作为异源表达系统的成功主要在于由于其高的体积生产率而获得了令人印象深刻的产量。然而，较低的单位生产率仍然阻碍了该平台的潜在成功。多(基因)复制克隆是一种潜在的快速和方便的提高重组蛋白效价的方法，但它们也不是没有陷阱。自从首次报道使用多拷贝克隆以来，已经有20多年的时间了，寻找最快、最有效的方法来产生这些菌株仍然是一个活跃的研究领域。也很明显，拷贝数和蛋白质效价之间并不总是线性相关，这导致了对如何将分泌压力的负面影响降至最低并实现克隆稳定性的深入研究。
The success of Pichia pastoris as a heterologous expression system lies predominantly in the impressive yields that can be achieved due to high volumetric productivity. However, low specific productivity still inhibits the potential success of this platform. Multi-(gene) copy clones are potentially a quick and convenient method to increase recombinant protein titer, yet they are not without their pitfalls. It has been more than twenty years since the first reported use of multi-copy clones and it is still an active area of research to find the fastest and most efficient method for generating these strains. It has also become apparent that there is not always a linear correlation between copy number and protein titer, leading to in-depth investigations into how to minimize the negative impact of secretory stress and achieve clonal stability.
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影响因子：
5
作者：
Lee, FWF;DaSilva, NA
通讯作者：
DaSilva, NA
影响因子：
4.4
作者：
Lee, Tae-Hee;Bae, Yi-Hyun;Seo, Jin-Ho
通讯作者：
Seo, Jin-Ho
影响因子：
46.9
作者：
De Schutter, Kristof;Lin, Yao-Cheng;Callewaert, Nico
通讯作者：
Callewaert, Nico
影响因子：
4.1
作者：
Mattanovich, D;Gasser, B;Sauer, M
通讯作者：
Sauer, M
影响因子：
3.8
作者：
Inan, M;Aryasomayajula, D;Meagher, MM
通讯作者：
Meagher, MM