Cloning and developmental expression of nonmuscle myosin IIA (Myh9) in the mammalian inner ear.
Cloning and developmental expression of nonmuscle myosin IIA (Myh9) in the mammalian inner ear.
复制标题
哺乳动物内耳非肌肉肌球蛋白 IIA (Myh9) 的克隆和发育表达。
DOI：
10.1002/jnr.20065
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Lalwani,AnilK
中科院分区：
文献类型：
--
作者：
Mhatre,AnandN;Li,Jiang;Kim,Yuil;Coling,DonaldE;Lalwani,AnilK
编码非肌肉肌球蛋白重链的MYH 9与非综合征型和综合征型常染色体显性遗传性听力损失有关，提示这种运动蛋白在听觉器官中具有重要的生物学作用。虽然Myh9表达已在成年小鼠中描述，但与其发育表达有关的关键参数仍有待表征。目前的研究描述了小鼠Myh9 cDNA的克隆和Myh9在发育中的胎儿、新生儿和成人内耳中表达的时间起始和空间分布。克隆的Myh9 cDNA与已发表的基因组序列有两个单碱基对差异：T990C（G330G）和T5198A（L1733Q）。对胚胎（E15.5）和几个器官（包括耳蜗）的成人组织进行免疫印迹，鉴定出单一的250 kDa抗Myh9免疫反应条带，支持胎儿和成人中不存在Myh9剪接变体。原位表达分析确定Myh9分布在E10.5的耳囊上皮层内。发现Myh9表达在E13.5和E16.5时持续存在于耳蜗管周围的上皮内。在E16.5，发育中的耳蜗的感觉细胞对Myh9表达呈阳性。在新生儿和成人耳蜗，Myh 9的表达内观察到的感觉毛细胞和支持毛细胞的Corti器官，螺旋韧带，和螺旋利姆布斯，但不是在血管纹。Myh9在发育和成熟内耳中的鉴定表明这种蛋白质在听觉功能的发育和维持中的作用。© 2004 Wiley利斯公司
MYH9encoding a nonmuscle myosin heavy chain has been linked to nonsyndromic and syndromic forms of autosomal dominant hereditary hearing loss, suggesting a critical biological role of this motor protein in the auditory organ. While Myh9 expression has been described in the adult mouse, critical parameters pertaining to its developmental expression remain to be characterized. The current study describes cloning of the mouse Myh9 cDNA and the temporal onset and spatial distribution of Myh9 expression in the inner ear of the developing fetus, the neonate, and the adult. The cloned Myh9 cDNA contained two single‐base‐pair differences from the published genomic sequence: T990C (G330G) and T5198A (L1733Q). Immunoblotting of embryonic (E15.5) and adult tissues from several organs, including the cochlea, identified a single 250‐kDa anti‐Myh9‐immunoreactive band, supporting an absence of Myh9 splice variants in the fetus and the adult. In situ expression analysis identified Myh9 distributed within the epithelial layer of the otic vesicle at E10.5. Myh9 expression was found to persist within the epithelia surrounding the cochlear duct at E13.5 and E16.5. The sensory cells of the developing cochlea were positive for Myh9 expression at E16.5. Within the neonate and the adult cochlea, Myh9 expression was observed within the sensory hair cells and the supporting hair cells of the organ of Corti, the spiral ligament, and the spiral limbus, but not in the stria vascularis. Identification of Myh9 in the developing and mature inner ear suggests a role for this protein in the development and maintenance of auditory function. © 2004 Wiley‐Liss, Inc.
人 IL-2 基因启动子内的两个区域对于诱导型 IL-2 表达非常重要。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Williams,TM;Eisenberg,L;Burlein,JE;Norris,CA;Pancer,S;Yao,D;Burger,S;Kamoun,M;Kant,JA
通讯作者：
Kant,JA
人白细胞介素 2 基因的调控：激活 T 淋巴细胞中基因表达的 5 侧翼区域的功能性 DNA 序列
影响因子：
64.5
作者：
T. Fujita;H. Shibuya;T. Ohashi;K. Yamanishi;T. Taniguchi
通讯作者：
T. Taniguchi
溶剂对萤火虫荧光素酶催化活性的影响。
影响因子：
3.9
作者：
L. Kricka;M. De Luca
通讯作者：
M. De Luca