Fluorescence-based sensors to monitor localization and functions of linear and K63-linked ubiquitin chains in cells.
Fluorescence-based sensors to monitor localization and functions of linear and K63-linked ubiquitin chains in cells.
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DOI：
10.1016/j.molcel.2012.06.017
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发表时间：
2012-09-14
期刊：
影响因子：
16
通讯作者：
Dikic, Ivan
中科院分区：
文献类型：
--
作者：
van Wijk, Sjoerd J. L.;Fiskin, Evgenij;Putyrski, Mateusz;Pampaloni, Francesco;Hou, Jian;Wild, Philipp;Kensche, Tobias;Grecco, Hernan E.;Bastiaens, Philippe;Dikic, Ivan
泛素链修饰蛋白质组的主要子集，但由于缺乏适当的工具，泛素信号动力学和定位的检测是有限的。在这里，我们采用泛素结合域（UBD）为基础的荧光传感器监测线性和K63连接的链在体外和体内。我们利用NEMO和ABIN（UBAN）中的UBD检测线性链，并利用RAP 80泛素相互作用基序（UIM）和TAB 2 Npl 4锌指（NZF）结构域检测K63链。线性和K63传感器在细菌自噬过程中装饰了胞质沙门氏菌周围的泛素涂层，而K63传感器选择性地监测了固定和活细胞中帕金森诱导的线粒体自噬和DNA损伤反应。此外，线性和K63传感器可用于监测内源性信号传导途径，如通过其差异干扰TNF-和IL-1诱导的NF-κB途径的能力所证明的。我们建议，基于UBD的生物传感器可以作为原型，跟踪和跟踪其他链类型和泛素样信号在体内。
Ubiquitin chains modify a major subset of the proteome, but detection of ubiquitin signaling dynamics and localization is limited due to a lack of appropriate tools. Here, we employ ubiquitin-binding domain (UBD)-based fluorescent sensors to monitor linear and K63-linked chains in vitro and in vivo. We utilize the UBD in NEMO and ABIN (UBAN) for detection of linear chains, and RAP80 ubiquitin-interacting motif (UIM) and TAB2 Npl4 zinc finger (NZF) domains to detect K63 chains. Linear and K63 sensors decorated the ubiquitin coat surrounding cytosolic Salmonella during bacterial autophagy, whereas K63 sensors selectively monitored Parkin-induced mitophagy and DNA damage responses in fixed and living cells. In addition, linear and K63 sensors could be used to monitor endogenous signaling pathways, as demonstrated by their ability to differentially interfere with TNF- and IL-1-induced NF-κB pathway. We propose that UBD-based biosensors could serve as prototypes to track and trace other chain types and ubiquitin-like signals in vivo.
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影响因子：
64.8
作者：
Ikeda, Fumiyo;Deribe, Yonathan Lissanu;Skanland, Sigrid S.;Stieglitz, Benjamin;Grabbe, Caroline;Franz-Wachtel, Mirita;van Wijk, Sjoerd J. L.;Goswami, Panchali;Nagy, Vanja;Terzic, Janos;Tokunaga, Fuminori;Androulidaki, Ariadne;Nakagawa, Tomoko;Pasparakis, Manolis;Iwai, Kazuhiro;Sundberg, John P.;Schaefer, Liliana;Rittinger, Katrin;Macek, Boris;Dikic, Ivan
通讯作者：
Dikic, Ivan
Nemo识别二丁素蛋白的结构基础。
影响因子：
16
作者：
Lo, Yu-Chih;Lin, Su-Chang;Rospigliosi, Carla C.;Conze, Dietrich B.;Wu, Chuan-Jin;Ashwell, Jonathan D.;Eliezer, David;Wu, Hao
通讯作者：
Wu, Hao
影响因子：
4.8
作者：
Dammer, Eric B.;Na, Chan Hyun;Peng, Junmin
通讯作者：
Peng, Junmin
影响因子：
56.9
作者：
Kim, Hongtae;Chen, Junjie;Yu, Xiaochun
通讯作者：
Yu, Xiaochun
影响因子：
16.6
作者：
Finley D
通讯作者：
Finley D