Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair
Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair
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自体间充质干细胞接种的纳米和微纤维脱细胞支架的制备用于腹股沟疝修补术
DOI：
10.2147/ijn.s125409
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发表时间：
2017-02
影响因子：
8
通讯作者：
Zhao Yilin
中科院分区：
文献类型：
--
作者：
Zhang Yinlong;Zhou Yuanyuan;Zhou Xu;Zhao Bin;Chai Jie;Liu Hongyi;Zheng Yifei;Wang Jinling;Wang Yaozong;Zhao Yilin
疝修补术中使用的补片通常伴有无血管纤维化瘢痕或补片收缩引起的慢性疼痛。在这项研究中，我们开发了一种组织工程网（TEM）通过种植自体骨髓来源的间充质干细胞到纳米纤维脱细胞主动脉（DA）。DA是通过依次用物理、机械、生物酶消化和化学洗涤剂方法使主动脉样品脱细胞来实现的。DA的三级结构由微米级、亚微米级和纳米级纤维构成，并保持了新鲜主动脉的原有强度。用TEM或脱细胞补片（AM）治疗腹股沟疝兔模型。植入后TEM组无疝复发，AM组腹股沟区有隆起，TEM组较AM组更厚、粘连更少、机械强度更强（P<0.05）。透射电镜显示细胞浸润、组织再生和新生血管形成较好（P<0.05）。因此，这些带有纳米纤维的细胞接种DA具有用于腹股沟疝修补术的潜力。
Prosthetic meshes used for hernioplasty are usually complicated with chronic pain due to avascular fibrotic scar or mesh shrinkage. In this study, we developed a tissue-engineered mesh (TEM) by seeding autologous bone marrow-derived mesenchymal stem cells onto nanosized fibers decellularized aorta (DA). DA was achieved by decellularizing the aorta sample sequentially with physical, mechanical, biological enzymatic digestion, and chemical detergent processes. The tertiary structure of DA was constituted with micro-, submicro-, and nanosized fibers, and the original strength of fresh aorta was retained. Inguinal hernia rabbit models were treated with TEMs or acellular meshes (AMs). After implantation, TEM-treated rabbit models showed no hernia recurrence, whereas AM-treated animals displayed bulges in inguinal area.At harvest, TEMs were thicker, have less adhesion, and have stronger mechanical strength compared to AMs (P<0.05). Moreover, TEM showed better cell infiltration, tissue regeneration,and neovascularization (P<0.05). Therefore,these cell-seeded DAs with nanosized fibers have potential for use in inguinal hernioplasty.
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影响因子：
2.4
作者：
Lai, JY;Chang, PY;Lin, JN
通讯作者：
Lin, JN
影响因子：
2.4
作者：
Fauza, DO;Marler, JJ;Vacanti, JP
通讯作者：
Vacanti, JP
影响因子：
9
作者：
J. Nathan;T. Pappas
通讯作者：
J. Nathan;T. Pappas
影响因子：
--
作者：
Diaz, Jose J., Jr.;Conquest, Anne M.;Donahue, Rafe
通讯作者：
Donahue, Rafe
影响因子：
3
作者：
Le, Dinh;Deveney, Clifford W.;Martindale, Robert G.
通讯作者：
Martindale, Robert G.