Clinical disease in sheep caused by bluetongue virus serotype 8, and prevention by an inactivated vaccine.
Clinical disease in sheep caused by bluetongue virus serotype 8, and prevention by an inactivated vaccine.
复制标题
蓝舌病毒血清型 8 引起的绵羊临床疾病及灭活疫苗的预防。
DOI：
10.1016/j.vaccine.2011.11.100
复制
发表时间：
2012
期刊：
影响因子：
5.5
通讯作者：
Moulin V
中科院分区：
文献类型：
--
作者：
Moulin V
能够减少临床症状，诱导中和抗体，也许最重要的是预防或减少病毒血症(从而减少病毒传播)的能力，是评估蓝舌病毒(BTV)疫苗效力的主要标准。因此，确定BTV挑战株--可靠地引起病毒血症的菌株和与自然感染动物相似的临床症状--对于疫苗评估是重要的。用BTV-8灭活疫苗(来自MSD Animal Health的‘Bovilis®BTV8’)免疫特克赛尔杂交羊和多塞特羊，用昆虫或哺乳动物细胞培养物中生长的低传代BTV-8(北欧株)进行攻击。记录临床症状的严重程度(使用改进的数字评分系统，如所述)以及病毒血症和血清中和抗体水平。在攻击时(接种疫苗后三周)检测到低水平的SN抗体。所有未接种疫苗的对照动物在攻击后均被感染，在攻击后21天(DPC)出现高滴度的SN抗体。接种者的SN抗体滴度上升较快，6dpc的滴度明显高于未接种的对照组。尽管只有有限的临床症状可以归因于感染哺乳动物细胞培养衍生病毒的年轻动物的BTV，但两种BTV-8挑战制剂都诱导了严重的临床症状，与在未接种疫苗的老年动物自然暴发期间观察到的蓝舌病相当。挑战在库蚊细胞培养中生长的BTV-8似乎比哺乳动物来源的BTV-8株引起更严重的临床评分和“死后损伤”。接种疫苗后，无论用哪种病毒制剂，都能很好地减少临床症状、发烧和病毒血症。
The ability to reduce clinical signs, induce neutralizing antibodies, and perhaps most importantly, to prevent or reduce viraemia (and therefore virus-transmission), represent primary criteria for assessment of bluetongue virus (BTV) vaccine efficacy. Identification of BTV challenge-strains that reliably induce viraemia and clinical signs comparable to those in naturally infected animals, is therefore important for vaccine evaluation. Texel cross-breed and Dorset Poll sheep vaccinated with inactivated BTV-8 vaccine (‘Bovilis®BTV8’ from MSD Animal Health), were challenged with low-passage BTV-8 (Northern European strain) grown in either insect (Culicoides) or mammalian cell-cultures. The severity of clinical signs was recorded (using a modified numerical scoring-system, which is described) along with viraemia and serum neutralizing (SN) antibody levels. Low level SN-antibodies were detected at the time of challenge (three weeks after vaccination). All unvaccinated control animals became infected after challenge, developing high SN-antibody titres by 21 days post challenge (dpc). Vaccinees showed faster increases in SN-antibody titres (‘booster’ response), with significantly higher titres at 6dpc than unvaccinated controls. Although only limited clinical-signs could be attributed to BTV in younger animals infected with the mammalian-cell-culture derived virus, both BTV-8 challenge preparations induced severe clinical signs comparable to ‘bluetongue’ observed during natural outbreaks in older unvaccinated animals. Challenge with BTV-8 grown in Culicoides cell-cultures seemed to induce greater severity of clinical-scores and ‘post-mortem lesions’ than the mammalian-derived BTV-8 strain. Vaccination reduced clinical signs, fever, and viraemia equally well after challenge with either virus preparation.
登录
查看更多内容
影响因子：
11.8
作者：
Toussaint JF;Sailleau C;Mast J;Houdart P;Czaplicki G;Demeestere L;VandenBussche F;van Dessel W;Goris N;Bréard E;Bounaadja L;Etienne T;Zientara S;De Clercq K
通讯作者：
De Clercq K
DOI：
10.1016/0147-9571(94)90044-2
发表时间：
1994-08-01
影响因子：
2
作者：
GIBBS, EPJ;GREINER, EC
通讯作者：
GREINER, EC
蓝舌病毒攻击实验中的污染。
影响因子：
5.5
作者：
M. Eschbaumer;R. Wäckerlin;G. Savini;S. Zientara;C. Sailleau;E. Bréard;M. Beer;B. Hoffmann
通讯作者：
B. Hoffmann
野毒株蓝舌病毒血清型 2 (BTV-2) 的 NS1 基因与减毒 BTV-2 疫苗的 NS1 基因之间的分子分化。
影响因子：
3.3
作者：
M. Agüero;M. Arias;L. Romero;M. J. Zamora;J. Sánchez
通讯作者：
J. Sánchez
2007 年荷兰奶牛群中蓝舌病血清型 8 病毒的垂直传播。
影响因子：
3.3
作者：
I. Santman;L. Wuijckhuise;P. Vellema;P. A. Rijn
通讯作者：
P. A. Rijn