Analysis of the origin and development of hatching gland cells by transplantation of the embryonic shield in the fish, Oryzias latipes
Analysis of the origin and development of hatching gland cells by transplantation of the embryonic shield in the fish, Oryzias latipes
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鱼胚胎盾移植分析孵化腺细胞的起源和发育
DOI：
10.1046/j.1440-169x.1999.00456.x
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发表时间：
1999
期刊：
影响因子：
4.6
通讯作者：
K. Yamagami
中科院分区：
文献类型：
--
作者：
K. Inohaya;S. Yasumasu;I. Yasumasu;I. Iuchi;K. Yamagami
观察到青冈的孵化腺细胞从原肠母细胞的前端分化出来，这似乎是在原肠形成开始时首先内卷的。这些结果表明，青竹的孵化腺细胞起源于胚盾，胚盾是这种鱼的假定组织者。目前的研究调查了孵化腺细胞是否真的起源于青竹的胚盾。进行了胚盾移植实验和孵化酶基因表达的原位杂交检测，作为腺细胞终末分化的标志。分析是按照以下过程进行的。首先，通过对青冈鹅类基因的表达及其组织体活性的测定，对胚盾区进行鉴定和功能鉴定。其次，证实了胚盾具有组织者活性，可诱导二次胚胎，并且初级胚胎和二次胚胎中孵化腺细胞的发育模式是相同的。最后，孵化酶基因表达鉴定的孵化腺细胞与移植胚盾的染料标记的后代细胞一致。综上所述，孵化的腺体细胞来源于青竹的胚盾，胚盾起着组织者的作用。
Hatching gland cells of the medaka, Oryzias latipes, have been observed to differentiate from the anterior end of the hypoblast, which seems to first involute at the onset of gastrulation. These results suggest that the hatching gland cells of medaka originate from the embryonic shield, the putative organizer of this fish. The present study investigated whether hatching gland cells really originate from the embryonic shield in the medaka. Transplantation experiments with embryonic shield and in situ hybridization detection of hatching enzyme gene expression as a sign of terminal differentiation of the gland cells were carried out. The analysis was performed according to the following processes. First, identification and functional characterization of the embryonic shield region were made by determining the expression of medaka goosecoid gene and its organizer activity. Second, it was confirmed that the embryonic shield had an organizer activity, inducing a secondary embryo, and that the developmental patterns of hatching gland cells in primary and secondary embryos were identical. Finally, the hatching gland cells as identified by hatching enzyme gene expression were found to coincide with the dye‐labeled progeny cells of the transplanted embryonic shield. In conclusion, it was determined that hatching gland cells were derived from the embryonic shield that functioned as the organizer in medaka.
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影响因子：
2.7
作者：
THISSE, C;THISSE, B;POSTLETHWAIT, JH
通讯作者：
POSTLETHWAIT, JH
影响因子：
2.6
作者：
Buchner, G;Broccoli, V;Franco, B
通讯作者：
Franco, B
斑马鱼goosecoid和无尾基因产物在野生型和突变型无尾胚胎中的表达。
DOI：
10.1242/dev.120.4.843
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Schulte-Merker,S;Hammerschmidt,M;Beuchle,D;Cho,KW;DeRobertis,EM;Nüsslein-Volhard,C
通讯作者：
Nüsslein-Volhard,C
影响因子：
4.6
作者：
C. Kimmel;R. M. Warga;T. Schilling
通讯作者：
C. Kimmel;R. M. Warga;T. Schilling
DOI：
10.1242/dev.122.7.2225
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Melby,AE;Warga,RM;Kimmel,CB
通讯作者：
Kimmel,CB