Transcriptome analysis of Azospirillum brasilense vegetative and cyst states reveals large-scale alterations in metabolic and replicative gene expression.
Transcriptome analysis of Azospirillum brasilense vegetative and cyst states reveals large-scale alterations in metabolic and replicative gene expression.
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DOI：
10.1099/mgen.0.000200
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发表时间：
2018-08
影响因子：
3.9
通讯作者：
Bauer CE
中科院分区：
文献类型：
--
作者：
Malinich EA;Bauer CE
几种革兰氏阴性土壤细菌在面对恶劣的环境条件时具有分化为休眠囊的能力。例如，当营养剥夺或干燥时，促进植物生长的细菌巴西偶氮螺旋菌从繁殖和活动的杆状营养细胞分化为不活动的休眠球形囊肿。目前，人们对营养细胞和囊肿细胞类型之间存在的代谢差异知之甚少，也不知道囊肿生理学方面允许休眠细胞在恶劣条件下存活。在这里，我们比较了无性和包囊性巴西孢子虫的转录组谱。我们观察到大约五分之一的巴西芽孢杆菌转录组在繁殖性营养细胞和非繁殖性囊肿之间的表达发生了变化。观察到细胞壁或细胞膜生物发生相关基因表达的显著变化，这与这些细胞类型之间发生的胞外多糖和脂质组成的变化是一致的。被包裹的细胞还表现出氨基酸生物合成、核糖体生物发生和翻译相关基因mRNA丰度的抑制。我们进一步观察到，囊肿创造了一个厌氧/微氧环境，氧化磷酸化基因的表达受到抑制，硝酸盐/亚硝酸盐还原和固氮基因的表达增加。
Several Gram-negative soil bacteria have the ability to differentiate into dormant cysts when faced with harsh environmental conditions. For example, when challenged with nutrient deprivation or desiccation, the plant-growth-promoting bacterium Azospirillum brasilense differentiates from a replicative and motile rod-shaped vegetative cell into a non-motile dormant spherical cyst. Currently, little is known about either the metabolic differences that exist between vegetative and cyst cell types, or about aspects of cyst physiology that allow dormant cells to survive harsh conditions. Here we compared transcriptomic profiles of vegetative and encysted A. brasilense. We observed that approximately one fifth of the A. brasilense transcriptome undergoes changes in expression between replicative vegetative cells and non-replicative cysts. A dramatic alteration in expression of genes involved in cell wall or cell membrane biogenesis was observed, which is congruent with changes in exopolysaccharide and lipid composition that occur between these cell types. Encysted cells also exhibited repressed mRNA abundance of genes involved in amino acid biosynthesis, ribosomal biogenesis and translation. We further observed that cysts create an anaerobic/micro-aerobic environment, as evidenced by repressed expression of oxidative phosphorylation genes coupled with increased expression of nitrate/nitrite reduction and nitrogen fixation genes.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
6.4
作者：
He K;Dragnea V;Bauer CE
通讯作者：
Bauer CE
影响因子：
11.3
作者：
Higgins D;Dworkin J
通讯作者：
Dworkin J
影响因子：
2.1
作者：
HAYWOOD, GW;ANDERSON, AJ;DAWES, EA
通讯作者：
DAWES, EA