Diphenylamine-2-carboxylate blocks Cl(-)-HCO3- exchange in Necturus gallbladder epithelium.
Diphenylamine-2-carboxylate blocks Cl(-)-HCO3- exchange in Necturus gallbladder epithelium.
复制标题
二苯胺-2-羧酸盐可阻断 Necturus 胆囊上皮细胞中的 Cl(-)-HCO3- 交换。
DOI：
10.1152/ajpcell.1987.253.1.c79
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Bazile,JE
中科院分区：
文献类型：
--
作者：
Reuss,L;Costantin,JL;Bazile,JE
应用细胞内微电极技术研究了二苯胺-2-羧酸盐（DPC）对河豚鱼胆囊上皮细胞离子转运的影响。在对照条件下，将DPC添加到粘膜浴溶液中引起两种细胞膜的浓度依赖性可逆超极化，且没有可测量的电阻变化。此外，DPC引起以下效应，均与顶端膜Cl（-）-HCO 3-交换的抑制一致：细胞内Cl-活性（aCli）下降，细胞内pH（pHi）升高，降低粘膜溶液[Cl-]产生的aCli和pHi变化减少，降低粘膜溶液[HCO 3-]产生的pHi变化减少。在茶碱处理的制剂类似的研究表明，DPC也抑制阴离子交换在这些条件下，但没有影响顶端膜电扩散Cl-渗透性引起的环AMP。在这些条件下，DPC引起细胞膜超极化，但对膜电阻的表观比率没有影响。此外，DPC对改变粘膜[Cl-]引起的顶端膜电压的快速变化没有影响，但对粘膜[Cl-]变化引起的较慢的二次电压变化以及降低粘膜[Cl-]引起的aCl i和pHi变化引起显著降低。因为其他人已经证明DPC阻断其他上皮细胞中的Cl-通道（Distefano，A.，M. Wittner，E. Schlatter，H. J. Lang，H. Englert和R.葛瑞格二苯胺-2-羧酸盐，Cl（-）-转运上皮细胞中Cl（-）-传导通路的阻断剂。Pfluegers++ Arch.405：S95-S100，1985），这些通道的结构可能与Necturus胆囊中由环AMP诱导的通道的结构不同。由于其相对较高的亲和力和快速可逆性，DPC可能成为其他细胞中阴离子交换研究的有用工具。
Intracellular microelectrode techniques were employed to study the effects of diphenylamine-2-carboxylate (DPC) on ion transport in Necturus gallbladder epithelium. Under control conditions, addition of DPC to the mucosal bathing solution caused a concentration-dependent, reversible hyperpolarization of both cell membranes with no measurable resistance changes. In addition, DPC caused the following effects, all consistent with inhibition of apical membrane Cl(-)-HCO3- exchange: fall in intracellular Cl- activity (aCli), increase in intracellular pH (pHi), reduction of the changes in aCli and pHi produced by lowering mucosal solution [Cl-], and reduction of the change in pHi produced by lowering mucosal solution [HCO3-]. Similar studies in theophylline-treated preparations indicate that DPC also inhibits anion exchange under these conditions, but has no effect on the apical membrane electrodiffusive Cl- permeability induced by cyclic AMP. Under these conditions, DPC caused cell membrane hyperpolarization but had no effect on the apparent ratio of membrane resistances. In addition, DPC had no effects on the rapid changes in apical membrane voltage elicited by altering mucosal [Cl-], but caused significant reductions of the slower, secondary voltage changes observed in response to changes in mucosal [Cl-], and the changes in aCli and pHi produced by lowering mucosal [Cl-]. Because others have demonstrated that DPC blocks Cl- channels in other epithelia (Distefano, A., M. Wittner, E. Schlatter, H. J. Lang, H. Englert, and R. Greger. Diphenylamine-2-carboxylate, a blocker of the Cl(-)-conductive pathway in Cl(-)-transporting epithelia. Pfluegers++ Arch. 405: S95-S100, 1985), it is possible that the structures of those channels and that induced by cyclic AMP in Necturus gallbladder are different. Because of its relatively high affinity and rapid reversibility, DPC may become useful in studies of anion exchange in other cells.
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阴离子两亲化合物抑制红细胞中的阴离子转运。
DOI：
--
发表时间：
1982
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
J. Cousin;R. Motais
通讯作者：
R. Motais
阴离子两亲化合物抑制红细胞中的阴离子转运。
DOI：
--
发表时间：
1982
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
J.;R. Motais
通讯作者：
R. Motais
H 和 HCO3- 在胆囊上皮盐转运中的作用。
影响因子：
18.2
作者：
Reuss,L;Stoddard,JS
通讯作者：
Stoddard,JS
影响因子：
5.5
作者：
HODGKIN, AL;HOROWICZ, P
通讯作者：
HOROWICZ, P