Biological Effects of Intravenous Vitamin C on Neutrophil Extracellular Traps and the Endothelial Glycocalyx in Patients with Sepsis-Induced ARDS.
Biological Effects of Intravenous Vitamin C on Neutrophil Extracellular Traps and the Endothelial Glycocalyx in Patients with Sepsis-Induced ARDS.
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静脉内维生素C对嗜中性粒细胞外陷阱和内皮糖脂的生物学作用对脓毒症诱导的ARDS患者的生物学作用。
DOI：
10.3390/nu14204415
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发表时间：
2022-10-21
期刊：
影响因子：
5.9
通讯作者：
Fowler AA
中科院分区：
文献类型：
--
作者：
Qiao X;Kashiouris MG;L'Heureux M;Fisher BJ;Leichtle SW;Truwit JD;Nanchal R;Hite RD;Morris PE;Martin GS;Sevransky J;Fowler AA
(1)背景：大剂量静脉注射维生素C （HDIVC）治疗脓毒症引起的急性呼吸窘迫综合征（ARDS）的疾病改善机制尚不清楚。(2)方法：我们对随机安慰剂对照试验CITRIS-ALI的受试者进行了血浆生物标志物的事后研究。我们探讨了HDIVC对游离DNA （cfDNA）和syndecan-1的影响，它们分别是中性粒细胞胞外陷阱（NET）形成和内皮糖萼降解的替代物。(3)结果：167例受试者中，HDIVC组（84例）和安慰剂组（83例）cfDNA基线水平分别为2.18 ng/µL （SD 4.20 ng/µL）和2.65 ng/µL (SD 3.87 ng/µL), p = 0.45。48小时时，HDIVC组cfDNA减少量比安慰剂组高1.02 ng/µL, p = 0.05。HDIVC组和安慰剂组的平均基线syndecan-1水平分别为9.49 ng/mL （SD 5.57 ng/mL）和10.83 ng/mL (SD 5.95 ng/mL), p = 0.14。48小时时，安慰剂组的HDIVC组syndecan-1增加1.53 ng/mL (95% CI, 0.96 - 2.11)，而对照组为0.75 ng/mL （95% CI, 0.21 - 1.29, p = 0.05）。(4)结论：HDIVC输注降低了与败血症诱导的ARDS相关的无细胞DNA和syndecan-1生物标志物。这些生物标志物的改善分别表明NETosis和血管内皮糖萼脱落的改善。
(1) Background: The disease-modifying mechanisms of high-dose intravenous vitamin C (HDIVC) in sepsis induced acute respiratory distress syndrome (ARDS) is unclear. (2) Methods: We performed a post hoc study of plasma biomarkers from subjects enrolled in the randomized placebo-controlled trial CITRIS-ALI. We explored the effects of HDIVC on cell-free DNA (cfDNA) and syndecan-1, surrogates for neutrophil extracellular trap (NET) formation and degradation of the endothelial glycocalyx, respectively. (3) Results: In 167 study subjects, baseline cfDNA levels in HDIVC (84 subjects) and placebo (83 subjects) were 2.18 ng/µL (SD 4.20 ng/µL) and 2.65 ng/µL (SD 3.87 ng/µL), respectively, p = 0.45. At 48-h, the cfDNA reduction was 1.02 ng/µL greater in HDIVC than placebo, p = 0.05. Mean baseline syndecan-1 levels in HDIVC and placebo were 9.49 ng/mL (SD 5.57 ng/mL) and 10.83 ng/mL (SD 5.95 ng/mL), respectively, p = 0.14. At 48 h, placebo subjects exhibited a 1.53 ng/mL (95% CI, 0.96 to 2.11) increase in syndecan-1 vs. 0.75 ng/mL (95% CI, 0.21 to 1.29, p = 0.05), in HDIVC subjects. (4) Conclusions: HDIVC infusion attenuated cell-free DNA and syndecan-1, biomarkers associated with sepsis-induced ARDS. Improvement of these biomarkers suggests amelioration of NETosis and shedding of the vascular endothelial glycocalyx, respectively.
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影响因子：
4.5
作者：
Reitsma, Sietze;Slaaf, Dick W.;Vink, Hans;van Zandvoort, Marc A. M. J.;Egbrink, Mirjam G. A. oude
通讯作者：
Egbrink, Mirjam G. A. oude
影响因子：
3.7
作者：
Hirose T;Hamaguchi S;Matsumoto N;Irisawa T;Seki M;Tasaki O;Hosotsubo H;Yamamoto N;Yamamoto K;Akeda Y;Oishi K;Tomono K;Shimazu T
通讯作者：
Shimazu T
影响因子：
3.2
作者：
O'Brien XM;Biron BM;Reichner JS
通讯作者：
Reichner JS
影响因子：
2
作者：
Paul, Prabasaj;Pennell, Michael L.;Lemeshow, Stanley
通讯作者：
Lemeshow, Stanley
影响因子：
11.1
作者：
Rannikko, J.;Seiskari, T.;Aittoniemi, J.
通讯作者：
Aittoniemi, J.