Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.
Hand-held spectroscopic device for in vivo and intraoperative tumor detection: contrast enhancement, detection sensitivity, and tissue penetration.
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用于体内和术中肿瘤检测的手持光谱装置：对比度增强，检测灵敏度和组织穿透。
DOI：
10.1021/ac102058k
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发表时间：
2010-11-01
影响因子：
7.4
通讯作者：
Nie, Shuming
中科院分区：
文献类型：
--
作者：
Mohs, Aaron M.;Mancini, Michael C.;Singhal, Sunil;Provenzale, James M.;Leyland-Jones, Brian;Wang, May D.;Nie, Shuming
手术是治疗人类癌症最有效和最广泛使用的方法之一，但一个主要问题是外科医生经常不能切除整个肿瘤，留下肿瘤阳性边缘、转移性淋巴结和/或卫星肿瘤结节。在这里，我们报告使用手持式光谱笔设备（称为SpectroPen）和近红外造影剂进行术中恶性肿瘤检测，基于波长分辨测量的荧光或表面增强拉曼散射（SERS）信号。SpectroPen利用近红外二极管激光器（发射波长为785 nm）与紧凑的头部装置耦合，用于光激发和收集。这种笔形装置有效地从光纤中去除二氧化硅拉曼峰，并衰减反射的激发光，从而对荧光和拉曼信号进行敏感分析。其整体性能已通过荧光造影剂（吲哚菁绿，或ICG）和表面增强拉曼散射（SERS）造影剂（聚乙二醇胶体金）进行评估。在体外条件下，吲哚菁染料的检出限约为2-5 × 10−11 M， SERS造影剂的检出限约为0.5-1 × 10−13 M。离体组织渗透数据显示，当造影剂埋在新鲜动物组织中5-10 mm深时，荧光和拉曼信号减弱，但可分辨。携带生物发光4T1乳腺肿瘤的小鼠体内研究进一步证明，术前和术中可以精确检测肿瘤边界，对比信号与肿瘤生物发光密切相关。手术后，SpectroPen设备可以进一步评估手术腔周围的阳性和阴性肿瘤边缘，为实时肿瘤检测和图像引导手术提供了新的可能性。
Surgery is one of the most effective and widely used procedures in treating human cancers, but a major problem is that the surgeon often fails to remove the entire tumor, leaving behind tumor-positive margins, metastatic lymph nodes, and/or satellite tumor nodules. Here we report the use of a handheld spectroscopic pen device (termed SpectroPen) and near-infrared contrast agents for intraoperative detection of malignant tumors, based on wavelength-resolved measurements of fluorescence or surface-enhanced Raman scattering (SERS) signals. The SpectroPen utilizes a near-infrared diode laser (emitting at 785 nm) coupled to a compact head unit for light excitation and collection. This pen-shaped device effectively removes silica Raman peaks from the fiber optics and attenuates the reflected excitation light, allowing sensitive analysis of both fluorescence and Raman signals. Its overall performance has been evaluated by using a fluorescent contrast agent (indocyanine green, or ICG) as well as a surface-enhanced Raman scattering (SERS) contrast agent (pegylated colloidal gold). Under in vitro conditions, the detection limits are approximately 2–5 × 10−11 M for the indocyanine dye and 0.5–1 × 10−13 M for the SERS contrast agent. Ex vivo tissue penetration data show attenuated but resolvable fluorescence and Raman signals when the contrast agents are buried 5–10 mm deep in fresh animal tissues. In vivo studies using mice bearing bioluminescent 4T1 breast tumors further demonstrate that the tumor borders can be precisely detected preoperatively and intraoperatively, and that the contrast signals are strongly correlated with tumor bioluminescence. After surgery, the SpectroPen device permits further evaluation of both positive and negative tumor margins around the surgical cavity, raising new possibilities for real-time tumor detection and image-guided surgery.
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影响因子：
17.1
作者：
Liu J;Lau SK;Varma VA;Moffitt RA;Caldwell M;Liu T;Young AN;Petros JA;Osunkoya AO;Krogstad T;Leyland-Jones B;Wang MD;Nie S
通讯作者：
Nie S
影响因子：
3
作者：
Karni, Tami;Pappo, Itzhak;Lelcuk, Shlomo
通讯作者：
Lelcuk, Shlomo
影响因子：
4.9
作者：
Li, Shyh-Dar;Huang, Leaf
通讯作者：
Huang, Leaf
影响因子：
3.5
作者：
de Veld, DCG;Skurichina, M;Roodenburg, JLN
通讯作者：
Roodenburg, JLN
影响因子：
10.3
作者：
Dreher, MR;Liu, WG;Chilkoti, A
通讯作者：
Chilkoti, A