A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.
A versatile genome-scale PCR-based pipeline for high-definition DNA FISH.
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DOI:
10.1038/nmeth.2306
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发表时间:
2013-02
期刊:
影响因子:
48
通讯作者:
van Oudenaarden, Alexander
中科院分区:
文献类型:
--
作者:
Bienko, Magda;Crosetto, Nicola;Teytelman, Leonid;Klemm, Sandy;Itzkovitz, Shalev;van Oudenaarden, Alexander
We have developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters, and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genome readily usable for rapid and flexible generation of probes, making HD-FISH invaluable for many research and diagnostic applications.
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影响因子:
48
作者:
Raj, Arjun;van den Bogaard, Patrick;Rifkin, Scott A.;van Oudenaarden, Alexander;Tyagi, Sanjay
通讯作者:
Tyagi, Sanjay
DOI:
10.1159/000015390
发表时间:
1999-01-01
期刊:
CYTOGENETICS AND CELL GENETICS
影响因子:
--
作者:
Wiegant, JCAG;van Gijlswijk, RPM;Tanke, HJ
通讯作者:
Tanke, HJ
DOI:
10.1073/pnas.1213818110
发表时间:
2012-12-26
影响因子:
11.1
作者:
Beliveau, Brian J.;Joyce, Eric F.;Wu, Chao-ting
通讯作者:
Wu, Chao-ting
影响因子:
7
作者:
Rogan, PK;Cazcarro, PM;Knoll, JHM
通讯作者:
Knoll, JHM
影响因子:
5.8
作者:
Navin, Nicholas;Grubor, Vladimir;Wigler, Michael
通讯作者:
Wigler, Michael