Effect of sodium nitrite on ischaemia and reperfusion-induced arrhythmias in anaesthetized dogs: is protein S-nitrosylation involved?
Effect of sodium nitrite on ischaemia and reperfusion-induced arrhythmias in anaesthetized dogs: is protein S-nitrosylation involved?
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DOI：
10.1371/journal.pone.0122243
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Végh Á
中科院分区：
文献类型：
--
作者：
Kovács M;Kiss A;Gönczi M;Miskolczi G;Seprényi G;Kaszaki J;Kohr MJ;Murphy E;Végh Á
目的探讨无机亚硝酸盐对急性缺血再灌注性室性心律失常的保护作用。用氯氯蔗糖和氨基甲酸乙酯麻醉的狗，按两种方案静脉注射亚硝酸钠（0.2 μ mol -1min-1）。在方案1中，亚硝酸盐在冠状动脉左前降支（LAD）闭塞25分钟前10分钟和期间输注（NaNO2-PO, n = 14），而在方案2中，输注在闭塞血管再灌注前10分钟开始（NaNO2-PR, n = 12）。对照犬15只，给予生理盐水灌胃，进行相同时间的缺血再灌注。在整个实验过程中评估缺血和室性心律失常的严重程度，以及冠状窦血中血浆硝酸盐/亚硝酸盐（NOx）水平的变化。再灌注时测定心肌超氧化物和硝基酪氨酸（NT）水平。蛋白质s -亚硝基化（SNO）和s -谷胱甘肽化的变化也被检测。与对照组相比，阻断前或再灌注前使用亚硝酸钠可显著抑制阻断期间室性心律失常的数量和严重程度，并提高再灌注后的生存率（0% vs. 50%和92%）。亚硝酸盐处理犬的超氧化物和NT水平也显著降低。与对照组相比，仅在NaNO2-PR犬中发现SNO增加，而s -谷胱甘肽化主要发生在NaNO2-PO犬中。静脉输注亚硝酸盐可显著降低麻醉犬急性缺血再灌注引起的室性心律失常的严重程度。这种效应可能是由no介导的氧化应激减少引起的，可能是通过蛋白质SNO和/或s -谷胱甘肽酰化。
To provide evidence for the protective role of inorganic nitrite against acute ischaemia and reperfusion-induced ventricular arrhythmias in a large animal model. Dogs, anaesthetized with chloralose and urethane, were administered intravenously with sodium nitrite (0.2 µmolkg-1min-1) in two protocols. In protocol 1 nitrite was infused 10 min prior to and during a 25 min occlusion of the left anterior descending (LAD) coronary artery (NaNO2-PO; n = 14), whereas in protocol 2 the infusion was started 10 min prior to reperfusion of the occluded vessel (NaNO2-PR; n = 12). Control dogs (n = 15) were infused with saline and subjected to the same period of ischaemia and reperfusion. Severities of ischaemia and ventricular arrhythmias, as well as changes in plasma nitrate/nitrite (NOx) levels in the coronary sinus blood, were assessed throughout the experiment. Myocardial superoxide and nitrotyrosine (NT) levels were determined during reperfusion. Changes in protein S-nitrosylation (SNO) and S-glutathionylation were also examined. Compared with controls, sodium nitrite administered either pre-occlusion or pre-reperfusion markedly suppressed the number and severity of ventricular arrhythmias during occlusion and increased survival (0% vs. 50 and 92%) upon reperfusion. There were also significant decreases in superoxide and NT levels in the nitrite treated dogs. Compared with controls, increased SNO was found only in NaNO2-PR dogs, whereas S-glutathionylation occurred primarily in NaNO2-PO dogs. Intravenous infusion of nitrite profoundly reduced the severity of ventricular arrhythmias resulting from acute ischaemia and reperfusion in anaesthetized dogs. This effect, among several others, may result from an NO-mediated reduction in oxidative stress, perhaps through protein SNO and/or S-glutathionylation.
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影响因子：
37.8
作者：
Gonzalez, Felix M.;Shiva, Sruti;Arai, Andrew E.
通讯作者：
Arai, Andrew E.
DOI：
10.1073/pnas.0306706101
发表时间：
2004-03-23
影响因子：
11.1
作者：
Bryan, NS;Rassaf, T;Feelisch, M
通讯作者：
Feelisch, M
影响因子：
7.4
作者：
Iwase, Hirotaro;Robin, Emmanuel;Schumacker, Paul T.
通讯作者：
Schumacker, Paul T.
影响因子：
3.4
作者：
György, K;Végh, A;Parratt, JR
通讯作者：
Parratt, JR
影响因子：
3
作者：
HOELZER, M;SCHAAL, SF;LEIER, CV
通讯作者：
LEIER, CV