Biological assessment of a calcium silicate incorporated hydroxyapatite-gelatin nanocomposite: a comparison to decellularized bone matrix.
Biological assessment of a calcium silicate incorporated hydroxyapatite-gelatin nanocomposite: a comparison to decellularized bone matrix.
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DOI：
10.1155/2014/837524
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发表时间：
2014
影响因子：
--
通讯作者：
Ko CC
中科院分区：
文献类型：
--
作者：
Lee DJ;Padilla R;Zhang H;Hu WS;Ko CC
我们的实验室利用仿生学开发了一种基于羟基磷灰石-明胶-硅酸钙（HGCS）的合成骨支架。在这里，我们利用大鼠颅骨临界尺寸缺损（CSD）评估了 HGCS 支架在体内骨形成中的潜力。将 12 只 Sprague-Dawley 大鼠随机分为四组：对照组（仅存在缺陷）、脱细胞骨基质 (DECBM) 和带有或不带有多能成体祖细胞 (MAPC) 的 HGCS。使用 SDS 和 NH4OH 去除所有细胞来制备 DECBM。 12 周后，采集 CSD 标本以评估放射学、组织学和组织形态学结果。通过粘着斑、MTS和茜素红研究了材料的体外成骨作用。微型 CT 分析表明，DECBM 和 HGCS 支架组比其他组形成了更大的不透射线区域。使用组织学分析和荧光染料标记评估的骨再生在接种 MAPC 的 HGCS 支架中最高。 DECBM 组表现出有限的骨诱导性，导致植入物和宿主组织之间出现间隙。移植 HGCS+MAPC 的组产生的新骨形成量是原来的两倍，这似乎表明了有效骨再生的作用。总之，新型HGCS支架可以改善骨再生，是干细胞介导的骨再生的有前景的载体。
Our laboratory utilized biomimicry to develop a synthetic bone scaffold based on hydroxyapatite-gelatin-calcium silicate (HGCS). Here, we evaluated the potential of HGCS scaffold in bone formation in vivo using the rat calvarial critical-sized defect (CSD). Twelve Sprague-Dawley rats were randomized to four groups: control (defect only), decellularized bone matrix (DECBM), and HGCS with and without multipotent adult progenitor cells (MAPCs). DECBM was prepared by removing all the cells using SDS and NH4OH. After 12 weeks, the CSD specimens were harvested to evaluate radiographical, histological, and histomorphometrical outcomes. The in vitro osteogenic effects of the materials were studied by focal adhesion, MTS, and alizarin red. Micro-CT analysis indicated that the DECBM and the HGCS scaffold groups developed greater radiopaque areas than the other groups. Bone regeneration, assessed using histological analysis and fluorochrome labeling, was the highest in the HGCS scaffold seeded with MAPCs. The DECBM group showed limited osteoinductivity, causing a gap between the implant and host tissue. The group grafted with HGCS+MAPCs resulting in twice as much new bone formation seems to indicate a role for effective bone regeneration. In conclusion, the novel HGCS scaffold could improve bone regeneration and is a promising carrier for stem cell-mediated bone regeneration.
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影响因子：
14
作者：
Kang, Yunqing;Kim, Sungvvoo;Bishop, Julius;Khademhosseini, Ali;Yang, Yunzhi
通讯作者：
Yang, Yunzhi
影响因子：
3.6
作者：
Cooper GM;Mooney MP;Gosain AK;Campbell PG;Losee JE;Huard J
通讯作者：
Huard J
影响因子：
14
作者：
Lin, Hang;Yang, Guang;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.
DOI：
10.1007/s11420-005-0111-5
发表时间：
2005-09-01
期刊：
HSS journal : the musculoskeletal journal of Hospital for Special Surgery
影响因子：
--
作者：
Bostrom, Mathias P G;Seigerman, Daniel A
通讯作者：
Seigerman, Daniel A
影响因子：
14
作者：
Ng, Chee Ping;Sharif, Abdul Rahim Mohamed;Griffith, Linda G.
通讯作者：
Griffith, Linda G.