Quantification of HIV-1 RNA in plasma: comparable results with the NASBA HIV-1 RNA QT and the AMPLICOR HIV monitor test.
Quantification of HIV-1 RNA in plasma: comparable results with the NASBA HIV-1 RNA QT and the AMPLICOR HIV monitor test.
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血浆中 HIV-1 RNA 的定量：与 NASBA HIV-1 RNA QT 和 AMPLICOR HIV 监测测试的结果相当。
DOI：
10.1097/00042560-199610010-00003
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
J. Desmyter
中科院分区：
文献类型：
--
作者：
A. Vandamme;J. Schmit;S. Van Dooren;K. Van Laethem;E. Gobbers;W. Kok;P. Goubau;M. Witvrouw;W. Peetermans;E. De Clercq;J. Desmyter
我们调查和比较了两种商业试剂盒的重复性、准确性、检测限和动态范围，这些试剂盒使用了滴定的病毒库(实验室株HIV-1 IIIB)和25名HIV-1感染患者的107份血浆样本来定量测定RNA病毒载量。这两种方法的高重复性(两种方法的SD=0.2-0.3log)允许可靠地检测到RNA病毒载量0.5log的变化。这两种方法具有相似的检测下限(至少10(3)个RNA拷贝/毫升血浆)和超过5对数(Amplicor)或6对数(NASBA)的HIV-1输入的动态范围。对于HIV-1 IIIB，病毒载量与病毒相关p24抗原的测量结果一致。对于21名患者(91个样本)，两种方法的RNA病毒载量相似，相差不超过0.5log。对于4名患者，对于所有16个样本，两种方法之间的病毒载量差异为>；0.5log。对于其中三名患者，这可以通过与Gag序列中的引物或探针不匹配来解释：这与病毒亚型没有相关性。与p24抗原测定相比，RNA病毒载量测定具有高度的敏感性(95%的患者有可检测到的病毒载量，而可检测到p24水平的患者为40%)，但在p24阳性样本中，抗原水平与RNA病毒载量之间的相关性仅具有边缘意义。我们还发现，全血中的病毒RNA在室温下运输过程中至少稳定了48小时。这些观察表明，NASBA HIV-1 RNA Qt测试和Amplicor HIV监测测试都是病毒载量的可靠参数，它们作为潜在的替代标记具有很大的前景。
We investigated and compared the reproducibility, accuracy, detection limits, and dynamic ranges of two commercial kits for quantification of RNA viral load using a titrated virus stock (laboratory strain HIV-1 IIIB) and 107 plasma samples of 25 HIV-1-infected patients. The high reproducibility of both methods (SD = 0.2-0.3 log for both methods) allowed reliable detection of a 0.5 log change in RNA viral load. Both methods had a similar detection limit (at least 10(3) RNA copies/ml plasma) and a dynamic range that extended over a 5 log (AMPLICOR) or a 6 log (NASBA) range of HIV-1 input. For HIV-1 IIIB, the viral load was compatible with measurements of virus-associated p24 antigen. For 21 patients (91 samples), the RNA viral load was similar with both methods differing by no more than 0.5 log. For four patients, the difference in viral load between the two methods was > 0.5 log for all 16 samples. For three of these patients, this could be explained by mismatches with primers or probes in the gag sequence: there was no correlation to the viral subtype. The RNA viral load determination was highly sensitive compared with p24 antigen measurement (> 95% of patients had a detectable viral load vs. 40% who had a detectable p24 level), but in the p24-positive samples the correlation between the antigen level and the RNA viral load was of only borderline significance. We also found that the viral RNA in whole blood was stable for at least 48 h during transport at room temperature. These observations show that both the NASBA HIV-1 RNA QT test and the AMPLICOR HIV monitor test are reliable parameters of the viral load, with great promise for their use as potential surrogate markers.
影响因子：
10.7
作者：
Andrey Zharkikh;Wen-Hsiung Li
通讯作者：
Andrey Zharkikh;Wen-Hsiung Li
影响因子：
56.9
作者：
PIATAK, M;SAAG, MS;LIFSON, JD
通讯作者：
LIFSON, JD
血浆人类免疫缺陷病毒 1 型 RNA 定量方法的多中心评价。
DOI：
10.1093/infdis/170.3.553
发表时间：
1994
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Lin,HJ;Myers,LE;Yen-Lieberman,B;Hollinger,FB;Henrard,D;Hooper,CJ;Kokka,R;Kwok,S;Rasheed,S;Vahey,M
通讯作者：
Vahey,M