In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter: possible role of this interaction in the expression of seed dormancy.
In vitro binding of Sorghum bicolor transcription factors ABI4 and ABI5 to a conserved region of a GA 2-OXIDASE promoter: possible role of this interaction in the expression of seed dormancy.
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DOI：
10.1093/jxb/ert347
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发表时间：
2013-12
影响因子：
6.9
通讯作者：
Rodríguez MV
中科院分区：
文献类型：
--
作者：
Cantoro R;Crocco CD;Benech-Arnold RL;Rodríguez MV
根据最终谷物用量精确调整休眠解除的时间对许多谷类作物来说仍然是一个挑战。粒用高粱[Sorghum bicolor（L.）Moench]在休眠水平和对收获前发芽（PHS）的敏感性方面表现出广泛的种内变异。胚对脱落酸（阿坝）和赤霉素（GA）代谢的敏感性在高粱籽粒发育的休眠性表达中起重要作用。在之前的工作中，研究表明，在胚胎对阿坝更敏感以及SbABA-INSTENSITIVE 4（SbABI 4）和SbABA-INSTENSITIVE 5（SbABI 5）表达更高的同时，休眠籽粒由于GA分解代谢更活跃而积累的活性较低的GA 4。在这项工作中，它表明，阿坝信号组分SbABI 4和SbABI 5在体外与SbGA 2-氧化酶3（SbGA 2 ox 3）启动子的片段含有ABA响应复合物（ABRC）的相互作用。这两种转录因子能够结合的启动子，虽然不是同时，这表明它们可能竞争相同的顺式作用调控序列。这些相互作用在高粱籽粒休眠的表达中的生物学作用被提出：SbABI 4和/或SbABI 5在体内激活SbGA 2 ox 3基因的转录并促进SbGA 2 ox 3蛋白的积累;这将导致GA 4的主动降解，从而防止休眠籽粒的萌发。对单子叶植物和双子叶植物GA 2 oxs的5′-调控区进行了比较分析;来自二穗短柄草和水稻的近缘GA 2 oxs中ABRC的保守性表明，这些物种可能具有与高粱相同的调控机制。
The precise adjustment of the timing of dormancy release according to final grain usage is still a challenge for many cereal crops. Grain sorghum [Sorghum bicolor (L.) Moench] shows wide intraspecific variability in dormancy level and susceptibility to pre-harvest sprouting (PHS). Both embryo sensitivity to abscisic acid (ABA) and gibberellin (GA) metabolism play an important role in the expression of dormancy of the developing sorghum grain. In previous works, it was shown that, simultaneously with a greater embryo sensitivity to ABA and higher expression of SbABA-INSENSITIVE 4 (SbABI4) and SbABA-INSENSITIVE 5 (SbABI5), dormant grains accumulate less active GA4 due to a more active GA catabolism. In this work, it is demonstrated that the ABA signalling components SbABI4 and SbABI5 interact in vitro with a fragment of the SbGA 2-OXIDASE 3 (SbGA2ox3) promoter containing an ABA-responsive complex (ABRC). Both transcription factors were able to bind the promoter, although not simultaneously, suggesting that they might compete for the same cis-acting regulatory sequences. A biological role for these interactions in the expression of dormancy of sorghum grains is proposed: either SbABI4 and/or SbABI5 activate transcription of the SbGA2ox3 gene in vivo and promote SbGA2ox3 protein accumulation; this would result in active degradation of GA4, thus preventing germination of dormant grains. A comparative analysis of the 5′-regulatory region of GA2oxs from both monocots and dicots is also presented; conservation of the ABRC in closely related GA2oxs from Brachypodium distachyon and rice suggest that these species might share the same regulatory mechanism as proposed for grain sorghum.
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影响因子：
11.6
作者：
Casaretto, J;Ho, THD
通讯作者：
Ho, THD
DOI：
10.5772/53128
发表时间：
2013-01-01
期刊：
ABIOTIC STRESS - PLANT RESPONSES AND APPLICATIONS IN AGRICULTURE
影响因子：
--
作者：
Daszkowska-Golec, Agata;Szarejko, Iwona
通讯作者：
Szarejko, Iwona
影响因子：
7.2
作者：
Chandrasekharan, MB;Bishop, KJ;Hall, TC
通讯作者：
Hall, TC
影响因子：
7.2
作者：
FINKELSTEIN, RR
通讯作者：
FINKELSTEIN, RR
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS