An acoustofluidic scanning nanoscope using enhanced image stacking and processing.
An acoustofluidic scanning nanoscope using enhanced image stacking and processing.
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DOI：
10.1038/s41378-022-00401-2
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发表时间：
2022
影响因子：
7.9
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Jin, Geonsoo;Rich, Joseph;Xia, Jianping;He, Albert J.;Zhao, Chenglong;Huang, Tony Jun
大视场的纳米级光学分辨率是半导体制造、生物医学成像、纳米材料识别等许多研究和工业领域的重要特征。已经开发了几种扫描显微镜来解决分辨率和视场之间的逆关系；然而，这些扫描显微镜仍然依赖于荧光标记和复杂的光学系统。为了克服这些限制，我们开发了一种带有无缝图像合并算法(阿尔法混合过程)的双摄像头声流控纳米显微镜。这种设计使我们能够同时对样品和微球进行精确成像，并准确跟踪颗粒的路径和位置。因此，与以前的设计相比，使用我们的声流控扫描纳米显微镜捕获整个视场(200 × 200 μm)所需的图像数量减少了55倍。此外，通过应用阿尔法混合成像技术，图像质量也得到了极大的改善，这对于准确描述和识别纳米级物体或过程至关重要。这种双摄像头声流控纳米显微镜为高分辨率和大视场的增强纳米成像铺平了道路。
Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas, such as semiconductor fabrication, biomedical imaging, and nanoscale material identification. Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view; however, those scanning microscopes still rely upon fluorescence labeling and complex optical systems. To overcome these limitations, we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm (alpha-blending process). This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location. Therefore, the number of images required to capture the entire field of view (200 × 200 μm) by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs. Moreover, the image quality is also greatly improved by applying an alpha-blending imaging technique, which is critical for accurately depicting and identifying nanoscale objects or processes. This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.
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影响因子：
3.8
作者：
Chen, ZG;Taflove, A;Backman, V
通讯作者：
Backman, V
影响因子：
13.6
作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1098/rspa.2011.0269
发表时间：
2012-02-08
影响因子：
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作者：
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