Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome.
Experimental Chagas disease-induced perturbations of the fecal microbiome and metabolome.
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DOI：
10.1371/journal.pntd.0006344
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发表时间：
2018-03
影响因子：
3.8
通讯作者：
Siqueira-Neto JL
中科院分区：
文献类型：
--
作者：
McCall LI;Tripathi A;Vargas F;Knight R;Dorrestein PC;Siqueira-Neto JL
克氏锥虫寄生虫是恰加斯病的病原体。这些寄生虫感染心脏和胃肠道组织，导致局部炎症和组织损伤。消化性恰加斯病与食物吸收、肠道运输和排便的扰动有关。然而，T. Cruzi感染对肠道微生物群和代谢组的影响还有待表征。在这项研究中，我们应用基于质谱的代谢组学和16 S rRNA测序，在恰加斯病的小鼠模型中，通过急性期和慢性感染期，分析粪便细菌组成和粪便代谢组的感染相关变化。我们观察到与T.克氏感染这些变化包括共轭亚油酸（CLA）衍生物和瘤胃球菌科和毛螺菌科的特定成员的变化，以及二级胆汁酸和梭菌目成员的变化。这些结果突出了多“组学”和多微生物研究在了解一般寄生虫病，特别是恰加斯病的重要性。宿主-寄生虫相互作用通常作为二元系统进行研究，而不考虑宿主微生物群的作用。这项工作将微生物组研究整合到胃肠道恰加斯病的研究中。我们证明了T.克氏感染扰乱粪便微生物组和代谢组，表明影响胃肠腔的功能变化。我们的研究结果支持进一步调查的作用微生物-寄生虫相互作用在胃肠道恰加斯病的发病机制。
Trypanosoma cruzi parasites are the causative agents of Chagas disease. These parasites infect cardiac and gastrointestinal tissues, leading to local inflammation and tissue damage. Digestive Chagas disease is associated with perturbations in food absorption, intestinal traffic and defecation. However, the impact of T. cruzi infection on the gut microbiota and metabolome have yet to be characterized. In this study, we applied mass spectrometry-based metabolomics and 16S rRNA sequencing to profile infection-associated alterations in fecal bacterial composition and fecal metabolome through the acute-stage and into the chronic stage of infection, in a murine model of Chagas disease. We observed joint microbial and chemical perturbations associated with T. cruzi infection. These included alterations in conjugated linoleic acid (CLA) derivatives and in specific members of families Ruminococcaceae and Lachnospiraceae, as well as alterations in secondary bile acids and members of order Clostridiales. These results highlight the importance of multi-‘omics’ and poly-microbial studies in understanding parasitic diseases in general, and Chagas disease in particular. Host-parasite interactions are usually studied as a binary system, without considering the role of the host microbiota. This work integrates microbiome research into the study of gastrointestinal Chagas disease. We show that T. cruzi infection perturbs the fecal microbiome and metabolome, indicating functional changes affecting the gastrointestinal lumen. Our results support further investigation into the role of the microbiota-parasite interaction in gastrointestinal Chagas disease pathogenesis.
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影响因子：
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发表时间：
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