Molecular and developmental characterization of novel cDNAs of the myelin-associated/oligodendrocytic basic protein
Molecular and developmental characterization of novel cDNAs of the myelin-associated/oligodendrocytic basic protein
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髓磷脂相关/少突胶质细胞碱性蛋白新型 cDNA 的分子和发育特征
DOI：
10.1523/jneurosci.16-02-00467.1996
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发表时间：
1996
期刊：
影响因子：
6.4
通讯作者：
M. Schwab
中科院分区：
文献类型：
--
作者：
A. Holz;N. Schaeren;C. Schaefer;U. Pott;R. Colello;M. Schwab
本研究通过cDNA克隆鉴定了几种新的髓鞘相关/少突胶质细胞碱性蛋白（MOBP）亚型。它们是包含69、81和99个氨基酸（分别为8.2、9.7和11.7kDa）的小的、高度碱性的多肽，并且与所描述的蛋白质或结构域结构没有显示出显著的同源性。所有（迄今为止）鉴定的MOBP同种型在氨基酸1-68中是相同的，但在C-末端区域的长度和极性上不同。一种异构体，命名为MOBP 81，显示在发育过程中大量表达。有趣的是，MOBP 81在位置69-81处具有带正电荷的残基的显著聚类，这也是在髓鞘碱性蛋白（MBP）和Po中观察到的特征。如原位杂交所示，MOBP基因的表达发生在大鼠视神经发育过程中晚于MBP和蛋白脂质蛋白，正好与髓鞘致密化的开始相吻合。2.6 kb MOBP 81-A转录物定位于少突胶质细胞的突起中，而3.8 kb MOBP 81-B转录物限于核周区域。因此，MOBP 81-A和相关mRNA似乎被转运到少突胶质细胞的外周，如已知的MBP基因的转录物。MOBP基因的晚期发育表达表明MOBP蛋白在髓鞘形成的晚期步骤起作用，可能在髓鞘致密化和髓鞘的维持中起作用。
Several novel myelin-associated/oligodendrocytic basic protein (MOBP) isoforms were identified in this study by cDNA cloning. They are small, highly basic polypeptides comprising 69, 81, and 99 amino acids (8.2, 9.7, and 11.7 kDa, respectively) and show no significant homology with described proteins or domain structures. All (as yet) identified MOBP isoforms are identical in amino acids 1-68 but differ in the length and polarity of the C-terminal region. One isoform, designated MOBP81, was shown to be expressed abundantly during development. Interestingly, MOBP81 has a significant clustering of positively charged residues at positions 69-81, a feature that also has been observed for myelin basic protein (MBP) and Po. As demonstrated by in situ hybridization, MOBP gene expression occurs during development of the rat optic nerve later than that of MBP and proteolipid protein and coincides exactly with the beginning of myelin compaction. The 2.6 kb MOBP81-A transcript is localized in the processes of oligodendrocytes, whereas the 3.8 kb MOBP81-B transcript is restricted to the perinuclear region. Therefore, MOBP81-A and related mRNAs seem to be transported to the periphery of the oligodendrocytes, as is known for the transcripts of the MBP gene. The late developmental expression of the MOBP gene suggests that the MOBP proteins act at the late steps of myelin formation, possibly in myelin compaction and in the maintenance of the myelin sheath.
DOI：
10.1073/pnas.90.22.10695
发表时间：
1993-11-15
影响因子：
11.1
作者：
PRIBYL, TM;CAMPAGNONI, CW;CAMPAGNONI, AT
通讯作者：
CAMPAGNONI, AT
编码髓磷脂特异性蛋白的 mRNA 的空间分离。
DOI：
10.1073/pnas.84.21.7773
发表时间：
1987
影响因子：
11.1
作者：
Trapp,BD;Moench,T;Pulley,M;Barbosa,E;Tennekoon,G;Griffin,J
通讯作者：
Griffin,J
DOI：
10.1111/j.1432-1033.1986.tb10139.x
发表时间：
1986-11-17
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
WOODGETT, JR;GOULD, KL;HUNTER, T
通讯作者：
HUNTER, T