Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.
Merging of confocal and caging technologies: selective three-color communication with profluorescent reporters.
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DOI：
10.1002/anie.201202820
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发表时间：
2012-07-27
影响因子：
16.6
通讯作者：
Lawrence, David S.
中科院分区：
文献类型：
--
作者：
Priestman, Melanie A.;Shell, Thomas A.;Sun, Liang;Lee, Hsien-Ming;Lawrence, David S.
Light-cleavable agents (caged)[1, 2] have found utility in addressing fundamental issues in cell biology, most notably drawing correlations between intracellular biochemical activity and cell behavior. However, the current state of technology for caged compounds is generally restricted to functional groups that respond to long UV/short visible wavelengths (~ 360 nm). Recently, a few reports have appeared describing photolytic strategies that discriminate between two caged bioactive species.[3–7] The latter are based, in large part, on studies of photolabile protecting groups by Brochet [8] and Hagen [9]. These studies have laid the groundwork for a toolkit of wavelength distinguishable caging agents. We report the expansion of this toolkit to three wavelengths using the comparably weak (~ 30 kcal/mol) Co-alkyl moiety in alkyl-cobalamins [10, 11] as the light-cleavable bond.Barker and his coworkers described the photosensitivity of coenzyme B12 (where R= 5′-deoxyadenosyl) 50 years ago.[12] Alkylated cobalamins absorb light at 340–380 nm,~ 420 nm, and 500–570 nm. Illumination with light in any of these regions induces Co-alkyl bond scission with quantum yields on the order of 0.1–0.4.[13] To the best of our knowledge, the
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影响因子：
15
作者：
Pellois, JP;Hahn, ME;Muir, TW
通讯作者：
Muir, TW
影响因子：
15
作者：
Goguen, Brenda N.;Aemissegger, Andreas;Imperiali, Barbara
通讯作者：
Imperiali, Barbara
影响因子：
15
作者：
Kotzur, Nico;Briand, Benoit;Hagen, Volker
通讯作者：
Hagen, Volker
影响因子：
5.2
作者：
Smeltzer, CC;Cannon, MJ;Grissom, CB
通讯作者：
Grissom, CB
影响因子：
15
作者：
Lee, Hsien-Ming;Priestman, Melanie A.;Lawrence, David S.
通讯作者：
Lawrence, David S.