Miniaturized Chemical Tags for Optical Imaging.
Miniaturized Chemical Tags for Optical Imaging.
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DOI：
10.1002/anie.202204788
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发表时间：
2022-08-22
影响因子：
16.6
通讯作者：
Vendrell, Marc
中科院分区：
文献类型：
--
作者：
Benson, Sam;de Moliner, Fabio;Tipping, William;Vendrell, Marc
光学生物成像的最新进展促使人们需要最少的化学报告分子，这些报告分子可以保留生物分子的分子识别特性和活性特征。结果，将荧光和拉曼标记的尺寸减小到几个原子的几种方法（例如，单个芳基荧光团、拉曼活性三键和同位素）并将它们嵌入结构单元（例如，氨基酸、核碱基、糖）来构建天然样超分子结构。将小的光学报告分子整合到生物分子中也导致了智能分子实体，这些实体以前无法以快速的方式获得。在这篇文章中，我们回顾了最近的化学方法来合成小型化的光学标签，以及它们在生物成像中的一些多重应用。这篇小综述涵盖了用于光学生物成像的小化学标签的发展的一些最新进展，从它们的化学合成和表征到它们在不同生物系统中的应用，如细胞，组织和整个生物体。它侧重于荧光和拉曼标记的设计，并以代谢物，氨基酸，核酸以及细胞器和细胞靶向探针为特色。
Recent advances in optical bioimaging have prompted the need for minimal chemical reporters that can retain the molecular recognition properties and activity profiles of biomolecules. As a result, several methodologies to reduce the size of fluorescent and Raman labels to a few atoms (e.g., single aryl fluorophores, Raman‐active triple bonds and isotopes) and embed them into building blocks (e.g., amino acids, nucleobases, sugars) to construct native‐like supramolecular structures have been described. The integration of small optical reporters into biomolecules has also led to smart molecular entities that were previously inaccessible in an expedite manner. In this article, we review recent chemical approaches to synthesize miniaturized optical tags as well as some of their multiple applications in biological imaging. This Minireview covers some of the recent advances in the development of small chemical tags for optical bioimaging, from their chemical synthesis and characterization to their application in different biological systems, such as cells, tissues and whole organisms. It focuses on the design of fluorescent and Raman labels and features examples of metabolites, amino acids, nucleic acids as well as organelle and cell‐targeting probes.
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影响因子：
16.6
作者：
Chen C;Zhao Z;Qian N;Wei S;Hu F;Min W
通讯作者：
Min W
影响因子：
15
作者：
Chen, Zhixing;Paley, Daniel W.;Wei, Lu;Weisman, Andrew L.;Friesner, Richard A.;Nuckolls, Colin;Min, Wei
通讯作者：
Min, Wei
影响因子：
16.6
作者：
Du J;Su Y;Qian C;Yuan D;Miao K;Lee D;Ng AHC;Wijker RS;Ribas A;Levine RD;Heath JR;Wei L
通讯作者：
Wei L
影响因子：
4.9
作者：
Bell, Jonathan D.;Harkiss, Alexander H.;Sutherland, Andrew
通讯作者：
Sutherland, Andrew
影响因子：
--
作者：
Cho, Sung Ju;Ghorbani-Choghamarani, Arash;Hudson, Robert H E
通讯作者：
Hudson, Robert H E