A zinc finger protein BBX19 interacts with ABF3 to affect drought tolerance negatively in chrysanthemum

A zinc finger protein BBX19 interacts with ABF3 to affect drought tolerance negatively in chrysanthemum
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锌指蛋白 BBX19 与 ABF3 相互作用对菊花的耐旱性产生负面影响

DOI:
10.1111/tpj.14863
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发表时间:
2020-06
期刊:
影响因子:
7.2
通讯作者:
Hong Bo
Hong Bo
中科院分区:
生物学1区
文献类型:
--
作者:
Xu Yanjie;Zhao Xin;Aiwaili Palinuer;Mu Xianying;Zhao Meng;Zhao Jian;Cheng Lina;Ma Chao;Gao Junping;Hong Bo

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干旱是严重影响植物生长发育和分布的环境因素,严重影响农作物的产量和经济价值。我们从菊花抗旱基因转录组数据库中克隆了BBX家族IV亚组成员基因CmBBX19。干旱胁迫和ABA处理下调了CmBBX19的表达。我们构建了CmBBX19高表达系(CmBBX19-OX)和CmBBX19抑制系(CmBBX19-RNAi),发现与野生型(WT)对照相比,CmBBX19表达抑制导致了抗旱性的提高,而CmBBX19-OX的抗旱性降低。下游基因分析表明,CmBBX19主要通过诱导依赖于ABA的途径基因的表达变化来调节抗旱性,这些基因包括保护蛋白、氧化还原平衡和细胞壁生物发生基因,如对ABA 18、过氧化物酶12和纤维素合成酶类似蛋白G2的反应基因。此外,CmBBX19被证明与主要的ABA信号成分CmABF3相互作用,抑制这些下游基因的表达。我们认为BBX19-ABF3模块通过依赖于ABA的途径在菊花的耐旱性调控中发挥作用。
SUMMARY Drought is an environmental factor that can severely influence plant development and distribution, and greatly affect the yield and economic value of crops. We characterized CmBBX19, a BBX family subgroup IV member gene, from the transcriptome database of Chrysanthemum morifolium in response to drought stress. Drought stress and ABA treatments downregulated the expression of CmBBX19. We generated CmBBX19‐overexpressing (CmBBX19‐OX) lines and CmBBX19‐suppressing lines (CmBBX19‐RNAi), and found that suppressed expression of CmBBX19 led to enhanced drought tolerance compared with the wild‐type (WT) controls, while CmBBX19‐OX lines exhibited reduced drought tolerance. Downstream gene analysis showed that CmBBX19 modulates drought tolerance mainly through inducing changes in the expression of ABA‐dependent pathway genes, including protective protein, redox balance and cell wall biogenesis genes, such as responsive to ABA 18, peroxidase 12, and cellulose synthase‐like protein G2. Moreover, CmBBX19 was shown to interact with CmABF3, a master ABA signaling component, to suppress expression of these downstream genes. We conclude that BBX19‐ABF3 module functions in the regulation of drought tolerance of chrysanthemum through an ABA‐dependent pathway.
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发表时间: 2017-10-10
影响因子: 16.6
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发表时间: 2018-08-01
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