Control of chrysanthemum flowering through integration with an aging pathway.

Control of chrysanthemum flowering through integration with an aging pathway.
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通过与衰老途径整合来控制菊花开花

DOI:
10.1038/s41467-017-00812-0
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发表时间:
2017-10-10
影响因子:
16.6
通讯作者:
Gao J
Gao J
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Wei Q;Ma C;Xu Y;Wang T;Chen Y;Lü J;Zhang L;Jiang CZ;Hong B;Gao J

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年龄作为获得成花能力的一个阈值,在生物量不足时防止过早开花,并确保开花不受环境条件影响;然而,其潜在的调控机制在很大程度上是未知的。在本研究中,沉默短日照植物菊花(Chrysanthemum morifolium)的一个核因子基因CmNF - YB8的表达,无论日照长度条件如何,都会导致从幼年到成年的过早转变以及早开花。在CmNF - YB8 - RNAi植株中,SQUAMOSA启动子结合蛋白样(SPL)家族成员SPL3、SPL5和SPL9的表达上调,而微小RNA cmo - MIR156的表达下调。此外,CmNF - YB8被证明能与cmo - MIR156基因的启动子结合。利用基于病毒的微小RNA表达系统异位表达cmo - miR156,可恢复由CmNF - YB8沉默导致的早开花表型。这些结果表明,CmNF - YB8通过在衰老途径中直接调控cmo - MIR156的表达来影响开花时间。 植物年龄调控开花的机制仍未完全清楚。在这里作者表明,在菊花中,miR156对SPL转录因子的年龄依赖性调控通过控制NF - YB8的表达来影响开花。
Age, as a threshold of floral competence acquisition, prevents precocious flowering when there is insufficient biomass, and ensures flowering independent of environmental conditions; however, the underlying regulatory mechanisms are largely unknown. In this study, silencing the expression of a nuclear factor gene,CmNF-YB8, from the short day plant chrysanthemum (Chrysanthemum morifolium), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions. The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members,SPL3,SPL5, andSPL9, is upregulated inCmNF-YB8-RNAi plants, while expression of the microRNA,cmo-MIR156, is downregulated. In addition, CmNF-YB8 is shown to bind to the promoter of thecmo-MIR156gene. Ectopic expression of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotype caused byCmNF-YB8silencing. These results show that CmNF-YB8 influences flowering time through directly regulating the expression ofcmo-MIR156in the aging pathway.
DOI: 10.1073/pnas.1113971108
发表时间: 2011-10-18
影响因子: 11.1
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发表时间: 1998-12-01
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