Complete sequence and molecular epidemiology of IncK epidemic plasmid encoding blaCTX-M-14.
Complete sequence and molecular epidemiology of IncK epidemic plasmid encoding blaCTX-M-14.
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DOI：
10.3201/eid1704.101009
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发表时间：
2011-04
影响因子：
11.8
通讯作者：
Piddock LJ
中科院分区：
文献类型：
--
作者：
Cottell JL;Webber MA;Coldham NG;Taylor DL;Cerdeño-Tárraga AM;Hauser H;Thomson NR;Woodward MJ;Piddock LJ
该质粒在世界范围内传播于从人类和动物分离的大肠杆菌中。随着耐药菌株在世界范围内的出现，抗微生物药物耐药性是21世纪世纪的全球性挑战。由超广谱β-内酰胺酶（ESBLs）介导的对β-内酰胺类抗菌药物的可转移耐药性尤其令人关注。2004年，在英国从牛中分离出携带ESBL的IncK质粒（pCT）。该序列是一个93，629-bp的质粒，编码一个单一的抗微生物药物耐药基因blaCTX-M-14。根据这些信息，设计了鉴定pCT新特征的PCR，并应用于来自几个国家的分离株，表明该质粒已在世界范围内传播到人类和动物的细菌中。完整的DNA序列可以作为开发快速流行病学工具的平台，以识别和追踪临床相关病原体中质粒的传播，从而有助于更好地了解它们的分布和在人类和动物细菌之间转移的能力。
This plasmid is disseminated worldwide in Escherichia coli isolated from humans and animals. Antimicrobial drug resistance is a global challenge for the 21st century with the emergence of resistant bacterial strains worldwide. Transferable resistance to β-lactam antimicrobial drugs, mediated by production of extended-spectrum β-lactamases (ESBLs), is of particular concern. In 2004, an ESBL-carrying IncK plasmid (pCT) was isolated from cattle in the United Kingdom. The sequence was a 93,629-bp plasmid encoding a single antimicrobial drug resistance gene, blaCTX-M-14. From this information, PCRs identifying novel features of pCT were designed and applied to isolates from several countries, showing that the plasmid has disseminated worldwide in bacteria from humans and animals. Complete DNA sequences can be used as a platform to develop rapid epidemiologic tools to identify and trace the spread of plasmids in clinically relevant pathogens, thus facilitating a better understanding of their distribution and ability to transfer between bacteria of humans and animals.
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