Absence of SARS-CoV-2 Spike glycoprotein expression in placentas from individuals after mRNA SARS-CoV-2 vaccination.
Absence of SARS-CoV-2 Spike glycoprotein expression in placentas from individuals after mRNA SARS-CoV-2 vaccination.
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DOI：
10.1038/s41379-022-01061-3
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发表时间：
2022-09
期刊：
影响因子：
--
通讯作者：
Parra-Herran C
中科院分区：
文献类型：
--
作者：
Santos A;Sauer M;Neil AJ;Solomon IH;Hornick JL;Roberts DJ;Quade BJ;Parra-Herran C
目前遏制严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）全球大流行的公共卫生举措侧重于扩大疫苗接种工作，以包括孕妇等弱势群体。使用信使核糖核酸（mRNA）技术的疫苗依赖于免疫细胞的翻译，主要是在注射部位。一般人群对妊娠期间mRNA疫苗的安全性仍然犹豫不决，SARS-CoV-2刺突蛋白（现有mRNA疫苗的产物）是否在接种后在胎盘中积累仍然未知。目的：确定在妊娠期间最近的mRNA SARC-CoV-2疫苗接种背景下，胎盘组织中是否发生刺突蛋白翻译和积累。我们确定了48例在妊娠期间接受一剂或两剂mRNA SARS-CoV-2疫苗的患者，并在福尔马林固定、石蜡包埋的胎盘组织中对SARS-CoV-2刺突蛋白进行免疫组织化学。一个胎盘，阳性SARS-CoV-2 RNA原位杂交（ISH）被用作阳性对照。在SARS-CoV-2出现之前收集的7个足月胎盘作为阴性对照。研究组中81%的患者在妊娠晚期分娩，其余患者在妊娠早期自然流产或选择性妊娠中期终止妊娠。患者在怀孕期间接受两次（52%）或一次（48%）疫苗接种，最后一次接种与分娩之间的中位间隔为13天（范围2-79天）。大多数（63%）病例在分娩前15天内接受了最后一次剂量。所有研究组和阴性对照组的胎盘SARS-CoV-2免疫组化均为阴性。6例疫苗接种间隔较短（2-7天）的研究病例接受了SARS-CoV-2 ISH，结果为阴性。我们的研究结果表明，mRNA疫苗在胎盘中没有达到显着的浓度，因为胎盘组织中没有明确的SARS-CoV-2刺突蛋白积累。该观察结果提供了支持mRNA疫苗对胎盘-胎儿单位的安全性的证据。
Current public health initiatives to contain the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) global pandemic focus on expanding vaccination efforts to include vulnerable populations such as pregnant people. Vaccines using messenger ribonucleic acid (mRNA) technology rely on translation by immune cells, primarily at the injection site. Hesitancy remains among the general population regarding the safety of mRNA vaccines during gestation, and it remains unknown whether the SARS-CoV-2 Spike protein (the product of mRNA vaccines available) accumulates in the placenta after vaccination. Objective: To determine whether Spike protein translation and accumulation occurs in placental tissue in the context of recent mRNA SARC-CoV-2 vaccination during pregnancy. We identified 48 patients receiving one or two doses of mRNA SARS-CoV-2 vaccine during gestation and used immunohistochemistry against SARS-CoV-2 Spike protein in formalin-fixed, paraffin-embedded placental tissue. One placenta, positive for SARS-CoV-2 RNA by in situ hybridization (ISH) was used as positive control. Seven term placentas collected prior to the emergence of SARS-CoV-2 served as negative controls. Eighty one percent of patients in the study group underwent third-trimester delivery; remaining had a first-trimester spontaneous abortion or elective second-trimester termination. Patients received two (52%) or one (48%) vaccine doses during pregnancy, with a median interval between latest dose and delivery of 13 days (range 2–79 days). Most (63%) cases had their latest dose within 15 days prior to delivery. All the placentas in the study and negative control groups were negative for SARS-CoV-2 immunohistochemistry. Six study cases with short vaccine-delivery intervals (2–7 days) were subjected to SARS-CoV-2 ISH and were negative. Our findings suggest that mRNA vaccines do not reach significant concentrations in the placenta given the absence of definitive SARS-CoV-2 Spike protein accumulation in placental tissue. This observation provides evidence supporting the safety of mRNA vaccines to the placental-fetal unit.
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影响因子：
9.8
作者：
Gurol-Urganci I;Jardine JE;Carroll F;Draycott T;Dunn G;Fremeaux A;Harris T;Hawdon J;Morris E;Muller P;Waite L;Webster K;van der Meulen J;Khalil A
通讯作者：
Khalil A
影响因子：
13.8
作者：
Edlow AG;Li JZ;Collier AY;Atyeo C;James KE;Boatin AA;Gray KJ;Bordt EA;Shook LL;Yonker LM;Fasano A;Diouf K;Croul N;Devane S;Yockey LJ;Lima R;Shui J;Matute JD;Lerou PH;Akinwunmi BO;Schmidt A;Feldman J;Hauser BM;Caradonna TM;De la Flor D;D'Avino P;Regan J;Corry H;Coxen K;Fajnzylber J;Pepin D;Seaman MS;Barouch DH;Walker BD;Yu XG;Kaimal AJ;Roberts DJ;Alter G
通讯作者：
Alter G
影响因子：
6.3
作者：
Di Girolamo R;Khalil A;Alameddine S;D'Angelo E;Galliani C;Matarrelli B;Buca D;Liberati M;Rizzo G;D'Antonio F
通讯作者：
D'Antonio F
DOI：
10.1016/j.jconrel.2015.08.007
发表时间：
2015-11-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Pardi N;Tuyishime S;Muramatsu H;Kariko K;Mui BL;Tam YK;Madden TD;Hope MJ;Weissman D
通讯作者：
Weissman D
影响因子：
9.8
作者：
Roberts DJ;Edlow AG;Romero RJ;Coyne CB;Ting DT;Hornick JL;Zaki SR;Das Adhikari U;Serghides L;Gaw SL;Metz TD;National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development SARS-CoV-2 Placental Infection Workshop
通讯作者：
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health and Human Development SARS-CoV-2 Placental Infection Workshop