Regulatory T cell and activated natural killer cell infiltration in hepatocellular carcinoma: immune cell profiling using the CIBERSORT.
Regulatory T cell and activated natural killer cell infiltration in hepatocellular carcinoma: immune cell profiling using the CIBERSORT.
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肝细胞癌中的调节性 T 细胞和激活的自然杀伤细胞浸润：使用 CIBERSORT 进行免疫细胞分析
DOI：
10.21037/atm-20-5830
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发表时间：
2020-11
影响因子：
--
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Wang L;Yang Z;Cao Y
背景 肝细胞癌（HCC）被认为是由慢性肝病引起的免疫原性肿瘤。新兴研究表明各种免疫细胞和肿瘤细胞之间存在密切的相互作用。免疫表型分析对各种人类恶性肿瘤的预后显示出潜在的预测价值，可能允许根据免疫细胞浸润的程度和分布来识别有反应和无反应的患者。几种新型免疫治疗方法已经过试验并显示出有希望的疗效。然而，免疫疗法在 HCC 中的疗效受到多种因素的限制。本研究旨在研究肝癌中的肿瘤浸润免疫细胞。方法 通过估计 RNA 转录本的相对子集进行细胞类型识别 (CIBERSORT) 允许通过基因表达微阵列数据的去卷积进行免疫细胞分析。在本研究中，我们分析了从基因表达综合（GEO）数据库中的GSE84402获得的14对HCC组织样本中免疫细胞的比例。结果 在14对样本中，HCC组织显示出明显的调节性T细胞（Treg）、活化的自然杀伤（NK）细胞和M0巨噬细胞浸润（分别为P<0.001、P=0.007和P=0.001），并在CIBERSORT中进行了验证，P值设置为≤0.05。在 CIBERSORT 筛选出的四对样本中，发现与非肿瘤组织相比，HCC 组织具有显着的 Treg 细胞和活化的 NK 细胞浸润（分别为 P=0.007 和 P=0.015）。此外，Pearson相关分析显示Tregs与活化的NK细胞呈正相关（相关系数=0.41）。结论 HCC肿瘤组织中有Treg细胞和活化的NK细胞明显浸润，应作为HCC多学科治疗的候选治疗靶点。
Background Hepatocellular carcinoma (HCC) is understood to be an immunogenic tumor caused by chronic liver disease. Emerging research has indicated close interaction between various immune cells and tumor cells. Immunophenotyping, which has shown potential predictive value for the prognosis of various human malignancies, might allow responsive and non-responsive patients to be identified based on the extent and distribution of immune cell infiltration. Several novel immunotherapeutic approaches have been trialed and have shown promising efficacy. However, the efficacy of immunotherapies in HCC is limited by several factors. This study aimed to investigate tumor-infiltrating immune cells in HCC. Methods Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) allows immune cell profiling analysis by deconvolution of gene expression microarray data. In this study, we analyzed the proportions of immune cells in 14 paired samples of HCC tissues obtained from GSE84402 in Gene Expression Omnibus (GEO) database. Results In the 14 paired samples, HCC tissues showed significant infiltration by regulatory T cells (Tregs), activated natural killer (NK) cells, and M0 macrophages (P<0.001, P=0.007 and P=0.001, respectively), which were validated in CIBERSORT with the P value set at ≤0.05. In four paired samples identified from those selected by CIBERSORT, HCC tissues were found to have significant Treg and activated NK cell infiltration compared to non-tumor tissues (P=0.007 and P=0.015, respectively). Additionally, Pearson correlation analysis revealed Tregs to be positively correlated with activated NK cells (Correlation coefficient =0.41). Conclusions HCC tumor tissues were markedly infiltrated by Tregs and activated NK cells, which should be considered as candidate therapeutic targets in HCC multidisciplinary treatments.
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影响因子：
4.1
作者：
Liu P;Chen L;Zhang H
通讯作者：
Zhang H
DOI：
10.1002/hep.27822
发表时间：
2015-08
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Ji J;Eggert T;Budhu A;Forgues M;Takai A;Dang H;Ye Q;Lee JS;Kim JH;Greten TF;Wang XW
通讯作者：
Wang XW
影响因子：
7.3
作者：
Hagiwara, Yasuhiro;Ohashi, Yasuo;Tanaka, Masao
通讯作者：
Tanaka, Masao
趋化因子（C-X-C 基序）受体 3 阳性 B 细胞将白细胞介素 17 炎症与人肝细胞癌中促肿瘤巨噬细胞极化联系起来
影响因子：
13.5
作者：
Liu, Rui-Xian;Wei, Yuan;Kuang, Dong-Ming
通讯作者：
Kuang, Dong-Ming
影响因子：
4.6
作者：
Foerster F;Hess M;Gerhold-Ay A;Marquardt JU;Becker D;Galle PR;Schuppan D;Binder H;Bockamp E
通讯作者：
Bockamp E