Rice WRKY11 Plays a Role in Pathogen Defense and Drought Tolerance.
Rice WRKY11 Plays a Role in Pathogen Defense and Drought Tolerance.
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DOI：
10.1186/s12284-018-0199-0
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发表时间：
2018-01-12
期刊：
影响因子：
--
通讯作者：
Hwang DJ
中科院分区：
文献类型：
--
作者：
Lee H;Cha J;Choi C;Choi N;Ji HS;Park SR;Lee S;Hwang DJ
植物经常受到非生物和生物胁迫，而WRKY蛋白在对这种胁迫的响应中起着关键作用。OsWRKY11是由病原体、干旱和高温诱导的，表明在生物和非生物胁迫反应中具有功能。本研究鉴定为WRKY组IIc成员OsWRKY11。它是一种转录激活剂，定位于细胞核。异源表达OsWRKY11可提高水稻对水稻白叶枯病菌的抗性。在低表达OsWRKY11的转基因株系中，抗性受到抑制。OsWRKY11的异位表达导致防御相关基因的结构性表达，而OsWRKY11的敲除(Kd)在病原菌攻击过程中减少了防御相关基因的表达，这表明OsWRKY11激活了防御反应。OsWRKY11直接与几丁质酶2的启动子结合，并激活其转录。此外，OsWRKY11的异源表达提高了植物对干旱胁迫的耐受性，诱导了干旱反应基因的结构性表达。在OsWRKY11-kd植株中，干旱反应基因的诱导受到了影响。OsWRKY11还直接与干旱反应基因RAB21的启动子结合，激活其转录。此外，OsWRKY11蛋白水平受泛素-蛋白酶体系统控制。OsWRKY11通过正向调节生物和非生物胁迫相关基因的表达，整合了植物对病原体和非生物胁迫的反应。本文的在线版本(10.1186/s12284-0180199-0)包含补充材料，可供授权用户使用。
Plants are frequently subjected to abiotic and biotic stresses, and WRKY proteins play a pivotal role in the response to such stress. OsWRKY11 is induced by pathogens, drought, and heat, suggesting a function in biotic and abiotic stress responses. This study identified OsWRKY11, a member of WRKY group IIc. It is a transcriptional activator that localized to the nucleus. Ectopic expression of OsWRKY11 resulted in enhanced resistance to a bacterial pathogen, Xanthomonas oryzae pv. oryzae; resistance was compromised in transgenic lines under-expressing OsWRKY11. Ectopic expression of OsWRKY11 resulted in constitutive expression of defense-associated genes, whereas knock-down (kd) of OsWRKY11 reduced expression of defense-associated genes during pathogen attack, suggesting that OsWRKY11 activates defense responses. OsWRKY11 bound directly to the promoter of CHITINASE 2, a gene associated with defense, and activated its transcription. In addition, ectopic expression of OsWRKY11 enhanced tolerance to drought stress and induced constitutive expression of drought-responsive genes. Induction of drought-responsive genes was compromised in OsWRKY11-kd plants. OsWRKY11 also bound directly to the promoter of a drought-responsive gene, RAB21, activating its transcription. In addition, OsWRKY11 protein levels were controlled by the ubiquitin-proteasome system. OsWRKY11 integrates plant responses to pathogens and abiotic stresses by positively modulating the expression of biotic and abiotic stress-related genes. The online version of this article (10.1186/s12284-018-0199-0) contains supplementary material, which is available to authorized users.
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影响因子：
5.1
作者：
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