Expression of Bim, Noxa, and Puma in non-small cell lung cancer.
Expression of Bim, Noxa, and Puma in non-small cell lung cancer.
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DOI：
10.1186/1471-2407-12-286
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发表时间：
2012-07-12
期刊：
影响因子：
3.8
通讯作者：
Nishimura M
中科院分区：
文献类型：
--
作者：
Sakakibara-Konishi J;Oizumi S;Kikuchi J;Kikuchi E;Mizugaki H;Kinoshita I;Dosaka-Akita H;Nishimura M
Bcl-2蛋白家族的BH3-only成员被认为在细胞凋亡的控制和凋亡途径的启动中发挥关键作用。在这项研究中，我们评估了Bim、Noxa和Puma在非小细胞肺癌（NSCLC）中的表达。共对135例手术切除的非小细胞肺癌进行了Bim、Noxa和Puma表达的免疫组织化学评估。测定免疫评分，然后分析其与临床病理变量或生存结果的相关性。肿瘤细胞胞质中检测到Bim、Noxa和Puma的免疫组化反应性。Bim表达与多个临床病理因素相关，包括性别（p < 0.001）、吸烟习惯（p = 0.03）、病理组织学（p = 0.001）、病理T分期（p = 0.03）、病理疾病分期（p = 0.02）、肿瘤分化（p < 0.001）。多因素logistic回归分析显示，低表达Bim与鳞状细胞癌有显著相关性（p = 0.04），高表达Bim与高分化肿瘤有显著相关性（p = 0.02）。细胞生物学表达分析表明，低Bim表达与高Ki67之间存在联系。虽然Noxa的表达也被证明与吸烟习惯（p = 0.02）和病理组织学（p = 0.03）相关，但通过多因素logistic回归分析发现，其表达与临床特征之间没有很强的相关性。没有发现Puma表达与任何变量之间的相关性。我们的分析还表明BH3-only蛋白的表达水平与生存结果无关。目前的分析表明，Bim在非小细胞肺癌中的表达与鳞状细胞癌的组织学和肿瘤增殖有关。
The BH3-only members of the Bcl-2 protein family have been proposed to play a key role in the control of apoptosis and in the initiation of the apoptotic pathways. In this study, we evaluated the expression of Bim, Noxa, and Puma in non-small cell lung cancer (NSCLC). A total of 135 surgically resected NSCLCs were immunohistochemically assessed for Bim, Noxa, and Puma expression. The immunoscores were determined, and then its correlation with either the clinicopathological variables or the survival outcomes were analyzed. Immunohistochemical reactivity for Bim, Noxa, and Puma was detected in the cytoplasm of the tumor cells. Bim expression was associated with several clinicopathological factors, including sex (p < 0.001), smoking habit (p = 0.03), pathological histology (p = 0.001), pathological T stage (p = 0.03), pathological disease stage (p = 0.02), and differentiation of tumor (p < 0.001). Multivariate logistic regression analysis showed a significant correlation between low Bim expression and squamous cell carcinoma (p = 0.04), in addition to a correlation between high Bim expression and well differentiated tumors (p = 0.02). Analysis of cellular biological expression demonstrated a link between low Bim expression and high Ki67. While Noxa expression was also shown to be correlated with both smoking habit (p = 0.02) and the pathological histology (p = 0.03), there was no strong association observed between the expression and the clinical features when they were examined by a multivariate logistic regression analysis. No correlations were noted between Puma expression and any of the variables. Our analyses also indicated that the expression levels of the BH3-only proteins were not pertinent to the survival outcome. The current analyses demonstrated that Bim expression in the NSCLCs was associated with both squamous cell carcinoma histology and tumor proliferation.
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影响因子：
8
作者：
Ni Chonghaile T;Letai A
通讯作者：
Letai A
吉非替尼诱导的表达突变体EGFR的NSCLC细胞系杀死需要BIM，并且可以通过BH3 Mimetics增强。
影响因子：
15.8
作者：
Cragg MS;Kuroda J;Puthalakath H;Huang DC;Strasser A
通讯作者：
Strasser A
BIM的诱导对于突变体EGFR依赖性肺腺癌中EGFR激酶抑制剂触发的凋亡至关重要。
影响因子：
15.8
作者：
Gong, Yixuan;Somwar, Romel;Politi, Katerina;Balak, Marissa;Chmielecki, Juliann;Jiang, Xuejun;Pao, William
通讯作者：
Pao, William
在原发性切除的非小细胞肺癌中，Bcl-2家族的细胞凋亡调节蛋白和p53的表达。
影响因子：
8.8
作者：
Borner, MM;Brousset, P;Pfanner-Meyer, B;Bacchi, M;Vonlanthen, S;Hotz, MA;Altermatt, HJ;Schlaifer, D;Reed, JC;Betticher, DC
通讯作者：
Betticher, DC
影响因子：
11.2
作者：
Deng, Jing;Shimamura, Takeshi;Letai, Anthony
通讯作者：
Letai, Anthony