Expression of apoptosis regulatory proteins of the Bcl-2 family and p53 in primary resected non-small-cell lung cancer.
Expression of apoptosis regulatory proteins of the Bcl-2 family and p53 in primary resected non-small-cell lung cancer.
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在原发性切除的非小细胞肺癌中，Bcl-2家族的细胞凋亡调节蛋白和p53的表达。
DOI：
10.1038/sj.bjc.6690152
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发表时间：
1999-02
影响因子：
8.8
通讯作者：
Betticher, DC
中科院分区：
文献类型：
--
作者：
Borner, MM;Brousset, P;Pfanner-Meyer, B;Bacchi, M;Vonlanthen, S;Hotz, MA;Altermatt, HJ;Schlaifer, D;Reed, JC;Betticher, DC
Bcl-2家族的蛋白质以及p53是细胞凋亡的重要调节因子。这些蛋白质表达的变化可能会导致癌症的形成，并影响肿瘤对化疗和放疗的反应。我们使用人类Bcl-2、Mcl-1、Bax、巴克和p53蛋白的特异性抗体，在49例根治性切除的非小细胞肺癌（NSCLC）患者的存档标本中检测这些凋亡调节基因的表达。抗凋亡蛋白Bcl-2和Mcl-1免疫染色的肿瘤细胞分别存在于31%和58%的评估病例中，而促凋亡蛋白Bax和巴克的免疫阳性分别存在于47%和58%的样本中。p53免疫阳性率为61%。Bcl-2、p53与Mcl-1、Bax表达呈正相关（P= 0.02和P= 0.06），Bax与p53表达呈负相关（P= 0.008）。Bcl-2的表达对原发性切除的NSCLC患者的无复发生存率有负面影响（P= 0.02）。p53和Bcl-2的表达与无转移生存率显著相关（P< 0.01）。只有p53阳性肿瘤患者在随访期间发生转移。我们的研究结果证实了Bcl-2家族蛋白Bcl-2、Mcl-1、Bax和巴克在NSCLC中的频繁表达。可以预期，Bcl-2家族成员对这种疾病的临床结果没有直接的影响，因为它们在细胞凋亡调节中的相互作用是复杂的。© 1999癌症研究运动
Proteins of the Bcl-2 family as well as p53 are important regulators of apoptosis. Alterations in the expression of these proteins can contribute to the formation of cancer, as well as influence tumour response to chemo- and radiotherapy. We used antibodies specific for the human Bcl-2, Mcl-1, Bax, Bak and p53 proteins to examine the expression of these apoptosis-regulating genes in 49 archival specimens of patients with radically resected non-small-cell lung cancer (NSCLC). Tumour cells containing immunostaining for the antiapoptotic proteins Bcl-2 and Mcl-1 were present in 31% and 58% of the cases evaluated, respectively, whereas immunopositivity for the proapoptotic proteins Bax and Bak was found in 47% and 58% of the samples. p53 immunopositivity was detected in 61% of the samples. The expression of Bcl-2 and p53 and the expression of Mcl-1 and Bax showed a positive association (P= 0.02 and P= 0.06 respectively), whereas the expression of Bax was inversely related to p53 (P= 0.008). The expression of Bcl-2 had a negative influence on relapse-free survival in this population of primary resected NSCLC patients (P= 0.02). The expression of p53 and Bcl-2 was significantly associated with metastasis-free survival (P< 0.01). Only patients with p53-positive tumours developed metastases during the follow-up period. Our results establish the frequent expression of the Bcl-2 family proteins Bcl-2, Mcl-1, Bax and Bak in NSCLC. It can be expected that Bcl-2 family members have no straightforward impact on clinical outcome in this disease because their interactions in the regulation of apoptosis are complex. © 1999 Cancer Research Campaign
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影响因子：
4.8
作者：
Borner, C
通讯作者：
Borner, C
影响因子：
8.8
作者：
Fontanini, G;Vignati, S;Bigini, D;Mussi, A;Lucchi, M;Angeletti, C A;Basolo, F;Bevilacqua, G
通讯作者：
Bevilacqua, G
影响因子：
3.7
作者：
KAPLAN, EL;MEIER, P
通讯作者：
MEIER, P
影响因子：
56.9
作者：
HOLLSTEIN, M;SIDRANSKY, D;HARRIS, CC
通讯作者：
HARRIS, CC
影响因子：
45.3
作者：
Apolinario, RM;vanderValk, P;Giaccone, G
通讯作者：
Giaccone, G