Isolation and genome sequencing of cytomegaloviruses from Natal multimammate mice (Mastomys natalensis).
Isolation and genome sequencing of cytomegaloviruses from Natal multimammate mice (Mastomys natalensis).
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DOI：
10.1099/jgv.0.001873
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发表时间：
2023-08
影响因子：
3.8
通讯作者：
Davison, Andrew J.
中科院分区：
文献类型：
--
作者：
Hansen, Frederick;Vucak, Matej;Nichols, Jenna;Hughes, Joseph;Bane, Sidy;Camiolo, Salvatore;Filipe, Ana da Silva;Ostermann, Eleonore;Staliunaite, Laura;Chan, Baca;Mauch, Thekla;Sogoba, Nafomon;Streblow, Daniel N.;Voigt, Sebastian;Oestereich, Lisa;Ehlers, Bernhard;Redwood, Alec J.;Feldmann, Heinz;Brune, Wolfram;Rosenke, Kyle;Jarvis, Michael A.;Davison, Andrew J.
关键词：
不同的巨细胞病毒(CMV)广泛分布在其哺乳动物宿主中，具有高度宿主物种限制的模式。到目前为止，证明这一点的证据主要限于基于聚合酶链式反应的方法，目标是小的，保守的基因组区域，只有少数代表不同CMV物种的分离病毒的完整基因组被测序。我们现在已经将从组织中直接分离传染性病毒与完整的基因组测序相结合，以提供野生动物种群中CMV多样性的视图。我们的目标是Natal多哺乳类小鼠，这种小鼠在撒哈拉以南非洲很常见，已知携带多种人畜共患病病原体，被认为是拉萨病毒(LASV)蔓延到人类的主要来源。使用从纳塔尔分枝杆菌肾脏制备的转化上皮细胞，我们从马里野外研究地点37只动物中的14只(36 %)的唾液腺组织中分离出巨细胞病毒。基因组测序表明，这些初级分离株代表了三种不同的纳塔尔分枝杆菌CMV(MnatCMV：MnatCMV1、MnatCMV2和MnatCMV3)，其中一些动物携带多个MnatCMV或单个MNatCMV的多个毒株，可能是合并感染或重叠感染的结果。包括原始分离株和空斑纯化分离株，我们对两个MnatCMV1株(来自14个病毒测序)、6个MnatCMV2株(25个病毒)和10个MnatCMV3株(21个病毒)的基因组进行了测序和注释，共计18个MnatCMV株，共60个感染性病毒。系统发育分析表明，这些MnatCMV与鼠巨细胞病毒属(正疱疹病毒科、贝塔疱疹病毒亚科)中的其他小鼠巨细胞病毒同源，MNatCMV1和MnatCMV2之间的亲缘关系比MnatCMV3更近。MNatCMV分离株的可获得性及其基因组特征将作为基于MNatCMV的疫苗的产生的前奏，该疫苗针对纳塔尔分枝杆菌储藏库中的LASV。
Distinct cytomegaloviruses (CMVs) are widely distributed across their mammalian hosts in a highly host species-restricted pattern. To date, evidence demonstrating this has been limited largely to PCR-based approaches targeting small, conserved genomic regions, and only a few complete genomes of isolated viruses representing distinct CMV species have been sequenced. We have now combined direct isolation of infectious viruses from tissues with complete genome sequencing to provide a view of CMV diversity in a wild animal population. We targeted Natal multimammate mice (Mastomys natalensis), which are common in sub-Saharan Africa, are known to carry a variety of zoonotic pathogens, and are regarded as the primary source of Lassa virus (LASV) spillover into humans. Using transformed epithelial cells prepared from M. natalensis kidneys, we isolated CMVs from the salivary gland tissue of 14 of 37 (36 %) animals from a field study site in Mali. Genome sequencing showed that these primary isolates represent three different M. natalensis CMVs (MnatCMVs: MnatCMV1, MnatCMV2 and MnatCMV3), with some animals carrying multiple MnatCMVs or multiple strains of a single MnatCMV presumably as a result of coinfection or superinfection. Including primary isolates and plaque-purified isolates, we sequenced and annotated the genomes of two MnatCMV1 strains (derived from sequencing 14 viruses), six MnatCMV2 strains (25 viruses) and ten MnatCMV3 strains (21 viruses), totalling 18 MnatCMV strains isolated as 60 infectious viruses. Phylogenetic analysis showed that these MnatCMVs group with other murid viruses in the genus Muromegalovirus (subfamily Betaherpesvirinae, family Orthoherpesviridae), and that MnatCMV1 and MnatCMV2 are more closely related to each other than to MnatCMV3. The availability of MnatCMV isolates and the characterization of their genomes will serve as the prelude to the generation of a MnatCMV-based vaccine to target LASV in the M. natalensis reservoir.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1126/science.1185350
发表时间：
2010-04-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hansen SG;Powers CJ;Richards R;Ventura AB;Ford JC;Siess D;Axthelm MK;Nelson JA;Jarvis MA;Picker LJ;Früh K
通讯作者：
Früh K
DOI：
10.1099/jgv.0.001673
发表时间：
2021-10
期刊：
The Journal of general virology
影响因子：
--
作者：
Gatherer D;Depledge DP;Hartley CA;Szpara ML;Vaz PK;Benkő M;Brandt CR;Bryant NA;Dastjerdi A;Doszpoly A;Gompels UA;Inoue N;Jarosinski KW;Kaul R;Lacoste V;Norberg P;Origgi FC;Orton RJ;Pellett PE;Schmid DS;Spatz SJ;Stewart JP;Trimpert J;Waltzek TB;Davison AJ
通讯作者：
Davison AJ
影响因子：
64.8
作者：
Hansen, Scott G.;Ford, Julia C.;Lewis, Matthew S.;Ventura, Abigail B.;Hughes, Colette M.;Coyne-Johnson, Lia;Whizin, Nathan;Oswald, Kelli;Shoemaker, Rebecca;Swanson, Tonya;Legasse, Alfred W.;Chiuchiolo, Maria J.;Parks, Christopher L.;Axthelm, Michael K.;Nelson, Jay A.;Jarvis, Michael A.;Piatak, Michael, Jr.;Lifson, Jeffrey D.;Picker, Louis J.
通讯作者：
Picker, Louis J.
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.