COP1 promotes ABA-induced stomatal closure by modulating the abundance of ABI/HAB and AHG3 phosphatases.
COP1 promotes ABA-induced stomatal closure by modulating the abundance of ABI/HAB and AHG3 phosphatases.
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DOI：
10.1111/nph.17001
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Song CP
中科院分区：
文献类型：
--
作者：
Chen Q;Bai L;Wang W;Shi H;Ramón Botella J;Zhan Q;Liu K;Yang HQ;Song CP
植物气孔在叶片功能中起着至关重要的作用，控制水分蒸腾以响应环境胁迫并调节光合作用所需的气体交换。植物激素脱落酸（阿坝）促进气孔关闭并抑制光诱导的气孔开放。拟南芥E3泛素连接酶COP 1在阿坝介导的气孔关闭中发挥作用。然而，潜在的分子机制仍然没有完全理解。利用酵母双杂交技术鉴定了与COP 1相互作用的阿坝信号组分，并进行了生物化学、分子和遗传学研究，以阐明COP 1在阿坝信号转导中的调节作用。cop 1突变体在光照和黑暗条件下对阿坝触发的气孔关闭均不敏感。COP 1与拟南芥进化枝A型2C磷酸酶（PP 2Cs）ABI/HAB组和AHG 3相互作用并泛素化，从而触发它们的降解。脱落酸可增强COP 1介导的PP 2C降解。ABI 1和AHG 3的突变部分拯救了cop 1气孔表型和OST 1的磷酸化水平，OST 1是阿坝信号传导中的关键SnRK 2型激酶。我们的数据表明，COP 1是一种新的信号通路的一部分，通过调节进化枝A PP 2Cs的一个子集的稳定性来促进阿坝介导的气孔关闭。这些发现提供了新的见解阿坝和光信号成分之间的相互作用，在气孔运动的调制。
Plant stomata play a crucial role in leaf function, controlling water transpiration in response to environmental stresses and modulating the gas exchange necessary for photosynthesis. The phytohormone abscisic acid (ABA) promotes stomatal closure and inhibits light‐induced stomatal opening. The Arabidopsis thaliana E3 ubiquitin ligase COP1 functions in ABA‐mediated stomatal closure. However, the underlying molecular mechanisms are still not fully understood. Yeast two‐hybrid assays were used to identify ABA signaling components that interact with COP1, and biochemical, molecular and genetic studies were carried out to elucidate the regulatory role of COP1 in ABA signaling. The cop1 mutants are hyposensitive to ABA‐triggered stomatal closure under light and dark conditions. COP1 interacts with and ubiquitinates the Arabidopsis clade A type 2C phosphatases (PP2Cs) ABI/HAB group and AHG3, thus triggering their degradation. Abscisic acid enhances the COP1‐mediated degradation of these PP2Cs. Mutations in ABI1 and AHG3 partly rescue the cop1 stomatal phenotype and the phosphorylation level of OST1, a crucial SnRK2‐type kinase in ABA signaling. Our data indicate that COP1 is part of a novel signaling pathway promoting ABA‐mediated stomatal closure by regulating the stability of a subset of the Clade A PP2Cs. These findings provide novel insights into the interplay between ABA and the light signaling component in the modulation of stomatal movement.
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DOI：
10.1073/pnas.1908677116
发表时间：
2019-07-30
影响因子：
11.1
作者：
Julian, Jose;Coego, Alberto;Rodriguez, Pedro L.
通讯作者：
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影响因子：
27.5
作者：
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影响因子：
5.6
作者：
Guo S;Dai S;Singh PK;Wang H;Wang Y;Tan JLH;Wee W;Ito T
通讯作者：
Ito T
影响因子：
9.2
作者：
Duek, PD;Elmer, MV;Fankhauser, C
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影响因子：
27.5
作者：
Dong, Huan;Bai, Ling;Song, Chun-Peng
通讯作者：
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