Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co-Cultured With Mature Neuronal Cells.
Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co-Cultured With Mature Neuronal Cells.
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通过电刺激与成熟神经元细胞共培养的人间充质干细胞诱导神经发生。
DOI：
10.1002/mabi.201500115
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发表时间：
2015
影响因子：
4.6
通讯作者：
Keun‐Hong Park
中科院分区：
文献类型：
--
作者：
S. Park;J. S. Park;H. Yang;Se Won Yi;C. Kim;Keun‐Hong Park
为了电刺激人骨髓间充质干细胞，将金纳米颗粒涂覆在涂有聚乙烯亚胺的玻璃片上。检测脉冲和持续电刺激对人骨髓间充质干细胞的细胞毒作用和分化的影响。同时检测hMSCs与神经细胞共培养的效果。用RTPCR法和Western blotting法检测神经元特异性fic基因和蛋白的表达，以评价干细胞向神经元分化的情况。用扫描电子显微镜观察细胞的形态变化。与成熟神经元细胞共培养和电击刺激的人骨髓间充质干细胞显示出最大程度的突起生长(~gt；150 mm)和较小的胞体尺寸。
For electrical stimulation of hMSCs, gold nanoparticles were coated onto polyethyleneimine coated glass cover slips. The effects of pulsed or constant electrical stimulation upon cytotoxicity and differentiation of hMSCs were examined. The effects of co culturing hMSCs with neuronal cells were also tested. The neuronal differentiation of the stem cells was evaluated by determining the expression of neuron-specific genes and proteins using RT-PCR and Western blotting. Morphological changes were evaluated by scanning electron microscopy. The hMSCs co-cultured with mature neuronal cells and stimulated with electrical shock showed the greatest level of neurite outgrowth (>150 mm) and smaller cell body sizes.
影响因子：
14
作者：
Lee, Jae Y.;Bashur, Chris A.;Goldstein, Aaron S.;Schmidt, Christine E.
通讯作者：
Schmidt, Christine E.
影响因子：
14
作者：
Liang, Mao-Shih;Andreadis, Stelios T.
通讯作者：
Andreadis, Stelios T.
影响因子：
15.9
作者：
Volckaert, Thomas;Dill, Erik;De Langhe, Stijn P.
通讯作者：
De Langhe, Stijn P.