Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications.
Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications.
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DOI：
10.1016/j.biomaterials.2009.04.042
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
--
作者：
Lee, Jae Y.;Bashur, Chris A.;Goldstein, Aaron S.;Schmidt, Christine E.
关键词：
静电纺丝是一种很有前途的方法来创建能够支持粘附并引导神经元延伸用于神经再生的神经纤维结构。同时，在缺乏地形特征的情况下，神经元的电刺激也被证明可以引导轴突延伸。因此，本研究的目的是形成导电的电刺激结构，并检查电刺激结构和电刺激的组合效果。通过在随机和对齐的静电纺丝聚（乳酸-共-乙醇酸）（PLGA）纳米纤维上生长聚吡咯（PPy）来产生导电网，如通过扫描电子显微镜和X射线光子光谱所证实的。PPy-PLGA电纺网支持大鼠嗜铬细胞瘤12（PC 12）细胞和海马神经元的生长和分化，与未涂覆的PLGA对照网相当，这表明PPy-PLGA可能适合作为神经元组织支架的导电纳米纤维。电刺激研究表明，在PPy-PLGA支架上以10 mV/cm的电位刺激的PC 12细胞，与相同支架上未刺激的细胞相比，表现出40-50%的长神经突和40-90%的多神经突形成。此外，刺激排列的PPy-PLGA纤维上的细胞导致比随机PPy-PLGA纤维上的刺激更长的神经突和更多的神经突承载细胞，这表明电刺激和地形引导的组合效应以及这些支架用于神经组织应用的潜在用途。
Electrospinning is a promising approach to create nanofiber structures that are capable of supporting adhesion and guiding extension of neurons for nerve regeneration. Concurrently, electrical stimulation of neurons in the absence of topographical features also has been shown to guide axonal extension. Therefore, the goal of this study was to form electrically conductive nanofiber structures and to examine the combined effect of nanofiber structures and electrical stimulation. Conductive meshes were produced by growing polypyrrole (PPy) on random and aligned electrospun poly(lactic-co-glycolic acid) (PLGA) nanofibers, as confirmed by scanning electron micrographs and X-ray photon spectroscopy. PPy-PLGA electrospun meshes supported the growth and differentiation of rat pheochromocytoma 12 (PC12) cells and hippocampal neurons comparable to non-coated PLGA control meshes, suggesting that PPy-PLGA may be suitable as conductive nanofibers for neuronal tissue scaffolds. Electrical stimulation studies showed that PC12 cells, stimulated with a potential of 10 mV/cm on PPy-PLGA scaffolds, exhibited 40–50% longer neurites and 40–90% more neurite formation compared to unstimulated cells on the same scaffolds. In addition, stimulation of the cells on aligned PPy-PLGA fibers resulted in longer neurites and more neurite-bearing cells than stimulation on random PPy-PLGA fibers, suggesting a combined effect of electrical stimulation and topographical guidance and the potential use of these scaffolds for neural tissue applications.
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