Molecular characterization of a novel AP2 transcription factor ThWIND1-L from Thellungiella halophila

Molecular characterization of a novel AP2 transcription factor ThWIND1-L from Thellungiella halophila
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来自 Thellungiella halophila 的新型 AP2 转录因子 ThWIND1-L 的分子表征

DOI:
10.1007/s11240-012-0163-4
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发表时间:
2012-04
期刊:
Plant Cell, Tissue and Organ Culture: An International Journal On In Vitro Culture of Higher Plants
影响因子:
--
通讯作者:
Zhu, Jian
Zhu, Jian
中科院分区:
其他
文献类型:
--
作者:
Zhou, Cheng;Guo, Jiansheng;Feng, Zhenhua;Cui, Xianghuan;Zhu, Jian

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一些AP 2/ERF转录因子是创伤诱导的调节因子,在控制细胞去分化中起关键作用。在这项研究中,一个新的AP 2/ERF转录因子,WIND 1-like基因(ThWIND 1-L),分离和表征小盐芥。该cDNA克隆含有一个完整的开放阅读框架(ORF),全长1,068 bp,编码356个氨基酸。预测的ThWIND 1-L cDNA的氨基酸序列与拟南芥RAP2.4(WIND 1)具有高度的同源性。ThWIND 1-L的表达对创伤信号有快速的响应,在拟南芥植株中异位表达ThWIND 1-L,在没有外源激素的情况下促进愈伤组织的形成。此外,ThWIND 1-L的过表达影响了一些初级转录因子(TF),这些转录因子已被证明参与细胞脱分化和芽再生。这些结果表明,ThWIND 1-L可能是一种创伤诱导的调节因子,在细胞去分化的调控中起重要作用。
Several AP2/ERF transcription factors are wound-induced regulators, which play key roles in controlling cell dedifferentiation. In this study, a novel AP2/ERF transcription factor, the WIND1-like gene (ThWIND1-L), was isolated and characterized from Thellungiella halophila. ThWIND1-L cDNA clone contained a full open reading frame (ORF) of 1,068 bp encoding 356 amino acids. The predicted amino acid sequence of ThWIND1-L cDNA shared remarkably high degree of identity with the Arabidopsis RAP2.4 (WIND1). The expression of ThWIND1-L rapidly responded to the wound signals, ectopic expression of ThWIND1-L in Arabidopsis plants promoted callus tissue formation without the presence of exogenous hormones. Furthermore, overexpression of ThWIND1-L influenced some primary transcription factors (TFs), which have been shown to be involved in cell dedifferentiation and shoot regeneration. These results indicated ThWIND1-L might be a wound-induced regulator, which played an important role in the control of cell dedifferentiation.
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