Membrane current evoked by mitochondrial Na(+)-Ca(2+) exchange in mouse heart.
Membrane current evoked by mitochondrial Na(+)-Ca(2+) exchange in mouse heart.
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DOI：
10.1186/s12576-020-00752-3
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发表时间：
2020-04-30
影响因子：
2.3
通讯作者：
Matsuoka, Satoshi
中科院分区：
文献类型：
--
作者：
Islam, Mohammed M.;Takeuchi, Ayako;Matsuoka, Satoshi
线粒体Na ~+-Ca ~（2+）交换（NCXm）的产电性一直存在争议，至今未见膜电流的报道。我们首次成功地记录NCXm介导的电流使用来自小鼠心室的线粒体。在K+、Cl−和Ca 2+单向转运体电流被抑制的条件下，线粒体外Na+在移液管中用1 μM Ca 2+诱导内向电流。Na+的半最大浓度为35.6 mM。无Ca ~（2+）时，内向电流减弱，加入10 μM Ca ~（2+）时，内向电流增强。NCXm阻断剂CGP-37157可显著抑制Na+诱导的内向电流。然而，NCXm的反向模式，这应该被检测为外向电流，几乎没有引起线粒体外的Ca 2+与Na+在吸管中的应用。得出结论，NCXm是产电的。这种性质可能有利于促进Ca 2+从线粒体中挤出，线粒体具有大的负膜电位。
The electrogenicity of mitochondrial Na+–Ca2+ exchange (NCXm) had been controversial and no membrane current through it had been reported. We succeeded for the first time in recording NCXm-mediated currents using mitoplasts derived from mouse ventricle. Under conditions that K+, Cl−, and Ca2+ uniporter currents were inhibited, extra-mitochondrial Na+ induced inward currents with 1 μM Ca2+ in the pipette. The half-maximum concentration of Na+ was 35.6 mM. The inward current was diminished without Ca2+ in the pipette, and was augmented with 10 μM Ca2+. The Na+-induced inward currents were largely inhibited by CGP-37157, an NCXm blocker. However, the reverse mode of NCXm, which should be detected as an outward current, was hardly induced by extra-mitochondrial application of Ca2+ with Na+ in the pipette. It was concluded that NCXm is electrogenic. This property may be advantageous for facilitating Ca2+ extrusion from mitochondria, which has large negative membrane potential.
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影响因子：
5.8
作者：
Czyz, A;Kiedrowski, L
通讯作者：
Kiedrowski, L
DOI：
10.1096/fj.13-248161
发表时间：
2014-08
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Nita II;Hershfinkel M;Kantor C;Rutter GA;Lewis EC;Sekler I
通讯作者：
Sekler I
影响因子：
8.8
作者：
Kostic M;Ludtmann MH;Bading H;Hershfinkel M;Steer E;Chu CT;Abramov AY;Sekler I
通讯作者：
Sekler I
影响因子：
4.1
作者：
BRAND, MD
通讯作者：
BRAND, MD
影响因子：
5.5
作者：
Kim, Bongju;Matsuoka, Satoshi
通讯作者：
Matsuoka, Satoshi