Double-exclusive liquid repellency (double-ELR): an enabling technology for rare phenotype analysis.
Double-exclusive liquid repellency (double-ELR): an enabling technology for rare phenotype analysis.
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DOI：
10.1039/c8lc00584b
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发表时间：
2018-09-11
期刊：
影响因子：
6.1
通讯作者：
Beebe DJ
中科院分区：
文献类型：
--
作者：
Li C;Yu J;Paine P;Juang DS;Berry SM;Beebe DJ
双排他液体排斥性（双ELR）是一种极端的润湿性现象，其中相邻区域选择性地和完全地排斥非纹理化基底上具有不同表面化学性质的不混溶液体（即，没有微/纳米结构的衬底）。在双ELR条件下，每种液体与其非优选的表面化学性质没有物理接触（接触角为180°），因此能够完全分隔相邻的流体体积（例如，水和油之间）。这实现了一种新型的基于细胞培养的测定，其中来自常见故障模式的细胞损失（例如，由无意的细胞粘附、有害的水分损失/增加和液体处理死体积引起的生物污染）被显著减轻。重要的是，利用双ELR的原理来实现油下扫描图案化，即亚微升体积的水性介质（和细胞）的无损失、稳健和高通量分布。除了经由扫描图案化的高效分布之外，双ELR可以用于构造“模块化”（即，容易实现和/或与空间和时间控制连接在一起）用于体外模拟在细胞测定群体中特别感兴趣的生理学相关微环境的高阶结构，包括具有优异的空间和时间控制的多表型培养物，三维分层多表型培养物，具有细胞外基质的选择性机械线索的培养物（即，胶原纤维排列）和球状体培养物。总之，双ELR的这些特征独特地促进了有限细胞样品的培养和高含量分析（例如，几百到几千个细胞）。具有空间和时间分辨率的复杂细胞培养微环境通过双排他的液体排斥性和底油扫描图案化来实现。
Double-exclusive liquid repellency (double-ELR) is an extreme wettability phenomenon in which adjacent regions selectively and completely repel immiscible liquids with different surface chemistries on a non-textured substrate (i.e., a substrate in absence of micro/nano-structures). Under double-ELR conditions, each liquid exhibits no physical contact (contact angle of 180°) with its non-preferred surface chemistry, thus enabling complete partitioning of adjacent fluidic volumes (e.g., between water and oil). This enables a new type of cell culture-based assay, where cell loss from common failure modes (e.g., biofouling from inadvertent cell adhesion, detrimental moisture loss/gain, and liquid handling dead volumes) is significantly mitigated. Importantly, the principles of double-ELR were leveraged to achieve underoil sweep patterning, a no-loss, robust and high-throughput distribution of sub-microliter volumes of aqueous media (and cells). In addition to high-efficiency distribution via sweep patterning, double-ELR can be used to construct “modular” (i.e., easily implemented and/or linked together with spatial and temporal control) higher-order architectures for in vitro imitation of physiologically relevant microenvironments that are of particular interest within the cell assay community, including multi-phenotype cultures with excellent spatial and temporal control, three-dimensional layered multi-phenotype cultures, cultures with selective mechanical cues of extracellular matrix (i.e., collagen fiber alignment), and spheroid cultures. Together, these features of double-ELR uniquely facilitate culture and high content analysis of limited cellular samples (e.g., a few hundred to a few thousand cells). Complex cell-culture microenvironments with both spatial and temporal resolutions are enabled by double-exclusive liquid repellency and underoil sweep patterning.
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影响因子：
4.6
作者：
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DOI：
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发表时间：
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影响因子：
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