Characterization of Novel Ribosome-Associated Endoribonuclease SLFN14 from Rabbit Reticulocytes.
Characterization of Novel Ribosome-Associated Endoribonuclease SLFN14 from Rabbit Reticulocytes.
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DOI：
10.1021/acs.biochem.5b00302
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发表时间：
2015-06-02
期刊：
影响因子：
2.9
通讯作者：
Pisarev, Andrey V.
中科院分区：
文献类型：
--
作者：
Pisareva, Vera P.;Muslimov, Ilham A.;Tcherepanov, Andrew;Pisarev, Andrey V.
信使核糖核酸（mRNA）的周转是细胞控制基因表达的关键步骤。内切核糖核酸酶是一种在内部切割RNA分子的酶，是降解过程的一些关键组成部分。在此，我们详细描述了从兔网织红细胞裂解物中纯化的新型内切核糖核酸酶SLFN14。施拉芬（Schlafen）基因编码一个仅限于哺乳动物的蛋白质家族。它们的细胞功能未知或未被完全了解。在网织红细胞中，SLFN14强烈过表达，仅以短形式存在，全部附着于核糖体，并且似乎是主要的核糖体相关蛋白之一。SLFN14结合在核糖体和核糖体亚基的底部，切割RNA，但优先切割核糖体RNA（rRNA）和与核糖体相关的mRNA。这导致核糖体亚基的降解。这个过程严格依赖于镁离子（Mg²⁺）和锰离子（Mn²⁺），不依赖于三磷酸核苷（NTP），且无序列特异性。然而，在其他细胞类型中，SLFN14是一种全长的纯核蛋白，缺乏核糖体结合和核酸酶活性。突变分析揭示了核糖体结合位点以及对该蛋白质内切核酸酶活性至关重要的天冬氨酸。到目前为止，只有少数参与核糖体介导过程的内切核糖核酸酶得到了表征。此外，它们中没有一个被证明与核糖体直接相关。因此，我们的发现扩展了对参与哺乳动物翻译控制的内切核糖核酸酶的一般认识。
Turnover of mRNA is a critical step that allows cells to control gene expression. Endoribonucleases, enzymes cleaving RNA molecules internally, are some of the key components of the degradation process. Here we provide a detailed characterization of novel endoribonuclease SLFN14 purified from rabbit reticulocyte lysate. Schlafen genes encode a family of proteins limited to mammals. Their cellular function is unknown or incompletely understood. In reticulocytes, SLFN14 is strongly overexpressed, represented exclusively by the short form, all tethered to ribosomes, and appears to be one of the major ribosome-associated proteins. SLFN14 binds to ribosomes and ribosomal subunits in the low part of the body and cleaves RNA but preferentially rRNA and ribosome-associated mRNA. This results in the degradation of ribosomal subunits. This process is strictly Mg2+- and Mn2+-dependent, NTP-independent, and sequence nonspecific. However, in other cell types, SLFN14 is a full-length solely nuclear protein, which lacks ribosomal binding and nuclease activities. Mutational analysis revealed the ribosomal binding site and the aspartate essential for the endonucleolytic activity of protein. Only few endoribonucleases participating in ribosome-mediated processes have been characterized to date. Moreover, none of them are shown to be directly associated with the ribosome. Therefore, our findings expand the general knowledge of endoribonucleases involved in mammalian translation control.
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影响因子：
64.5
作者：
Gan, JH;Tropea, JE;Ji, XH
通讯作者：
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影响因子：
14.9
作者：
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通讯作者：
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DOI：
10.1016/j.bbrc.2009.08.094
发表时间：
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影响因子：
3.1
作者：
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通讯作者：
Majumdar, Adhip P. N.
DOI：
10.1016/j.bbrc.2008.03.032
发表时间：
2008-05-23
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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