Effects of Transforming Growth Factor β1 on Astroglial Cells in Culture
Effects of Transforming Growth Factor β1 on Astroglial Cells in Culture
复制标题
转化生长因子β1对培养的星形胶质细胞的影响
作者：
D. Toru‐Delbauffe;D. Baghdassarian;J. Gavaret;F. Courtin;M. Pomérance;M. Pierre
摘要：探讨转化生长因子β1(TGF1)对无血清培养的星形胶质细胞β合成及功能分化的影响。转化生长因子β-1抑制和延迟血清诱导的DNA合成高峰。转化生长因子β-1处理组的细胞体积大于对照组。这一因素推迟了酸性成纤维细胞生长因子诱导的突起细胞的出现，也影响了星形胶质细胞特异性酶谷氨酰胺合成酶(GS)，其积累受氢化可的松(HC)控制。转化生长因子β-1以剂量和时间依赖的方式抑制HC诱导的GS活性。此外，转化生长因子β-1处理4h后，˜活性在去除该因子后16h仍保持抑制状态。这些结果提示转化生长因子β-1可能是星形胶质细胞生长和分化的重要调节因子。
Abstract: The effects of transforming growth factor β1 (TGFβ1) on DNA synthesis and functional differentiation of astroglial cells cultured in serum‐free medium were investigated. TGFβ1 diminished and delayed the peak of DNA synthesis induced by serum. TGFβ1‐treated cells were larger than control cells. This factor delayed the appearance of process‐bearing cells induced by acidic fibroblast growth factor treatment and also affected the astrocyte‐specific enzyme glutamine synthetase (GS), whose accumulation is under hydrocortisone (HC) control. TGFβ1 inhibited the induction of GS activity by HC in a dose‐ and time‐dependent manner. Moreover, pretreatment with TGFβ1 for 4 h maintained the inhibition of GS activity for ˜16 h after removal of this factor from culture medium. These results suggest that TGFβ1 may be an important regulator of astrocyte growth and differentiation.
DOI：
--
发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
雪旺细胞衍生的基底层和层粘连蛋白基质上中枢神经系统神经元的神经突生长的年龄依赖性模式和速率。
影响因子：
2.9
作者：
Schinstine,M;Cornbrooks,CJ
通讯作者：
Cornbrooks,CJ