Integrins Were Involved in Soybean Agglutinin Induced Cell Apoptosis in IPEC-J2.
Integrins Were Involved in Soybean Agglutinin Induced Cell Apoptosis in IPEC-J2.
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IPEC-J2 中整合素参与大豆凝集素诱导的细胞凋亡
DOI：
10.3390/ijms19020587
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发表时间：
2018-02-16
影响因子：
5.6
通讯作者：
Qin G
中科院分区：
文献类型：
--
作者：
Pan L;Zhao Y;Farouk MH;Bao N;Wang T;Qin G
大豆凝集素（soybean agglutinin，SBA）是一种非纤维碳水化合物相关蛋白质，是一种主要的抗营养因子。整合素是一种跨膜糖蛋白，参与许多生物学过程。虽然最近的工作表明，整合素参与SBA诱导的细胞周期的改变，没有全面的研究报告是否整合素参与SBA诱导的细胞凋亡（SCA）在IPEC-J2。SBA与整合素的关系尚不清楚。本研究旨在阐明SBA对IPEC-J2细胞增殖和细胞凋亡的影响;研究整合素在IPEC-J2正常细胞凋亡（NCA）和SCA中的作用;阐明SBA与整合素之间的关系和联系类型。因此，用0、0.125、0.25、0.5、1.0或2.0 mg/mL水平的SBA处理IPEC-J2细胞以测定细胞增殖和细胞凋亡。将细胞分为对照组、SBA处理组、整联蛋白抑制剂组和SBA +整联蛋白抑制剂组以确定整联蛋白在SCA中的功能。结果表明，SBA能显著抑制IPEC-J2细胞的增殖（p < 0.05），诱导细胞凋亡（p < 0.05）。抑制任何类型的整合素均可诱导细胞凋亡（p < 0.05），这些整合素参与SCA（p < 0.05）。即使SBA与整合素没有物理联系，也检测到SBA与α-辅肌动蛋白-2 ACTN 2（整合素结合蛋白）之间的关联。此外，SBA通过下调ACTN 2的基因表达水平来降低整合素的mRNA表达。我们的结论是，SBA的抗营养机制的证据，通过ACTN 2与整合素。需要进一步的试验来证明ACTN 2是否是连接SBA与整合素的唯一蛋白。
Soybean agglutinin (SBA), is a non-fiber carbohydrate related protein and a major anti-nutritional factor. Integrins, transmembrane glycoproteins, are involved in many biological processes. Although recent work suggested that integrins are involved in SBA-induced cell-cycle alterations, no comprehensive study has reported whether integrins are involved in SBA-induced cell apoptosis (SCA) in IPEC-J2. The relationship between SBA and integrins are still unclear. We aimed to elucidate the effects of SBA on IPEC-J2 cell proliferation and cell apoptosis; to study the roles of integrins in IPEC-J2 normal cell apoptosis (NCA) and SCA; and to illustrate the relationship and connection type between SBA and integrins. Thus, IPEC-J2 cells were treated with SBA at the levels of 0, 0.125, 0.25, 0.5, 1.0 or 2.0 mg/mL to determine cell proliferation and cell apoptosis. The cells were divided into control, SBA treated groups, integrin inhibitor groups, and SBA + integrin inhibitor groups to determine the integrin function in SCA. The results showed that SBA significantly (p < 0.05) lowered cell proliferation and induced cell apoptosis in IPEC-J2 (p < 0.05). Inhibition of any integrin type induced the cell apoptosis (p < 0.05) and these integrins were involved in SCA (p < 0.05). Even SBA had no physical connection with integrins, an association was detected between SBA and α-actinin-2 ACTN2 (integrin-binding protein). Additionally, SBA reduced the mRNA expression of integrins by down regulating the gene expression level of ACTN2. We concluded an evidence for the anti-nutritional mechanism of SBA by ACTN2 with integrins. Further trials are needed to prove whether ACTN2 is the only protein for connecting SBA with integrin.
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