Optimization of real-time analysis of sleep-wake cycle in mice.

Optimization of real-time analysis of sleep-wake cycle in mice.
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DOI:
10.1016/j.mex.2022.101811
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发表时间:
2022
期刊:
影响因子:
1.9
通讯作者:
Gerashchenko, Dmitry
Gerashchenko, Dmitry
中科院分区:
其他
文献类型:
--
作者:
Thankachan, Stephen;Gerashchenko, Andrei;Kastanenka, Ksenia, V;Bacskai, Brian J.;Gerashchenko, Dmitry

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Studying the biology of sleep requires accurate and efficient assessment of the sleep stages. However, analysis of sleep-wake cycles in mice and other laboratory animals remains a time-consuming and laborious process. In this study, we developed a Python script and a process for the streamlined analysis of sleep data that includes real-time processing of electroencephalogram (EEG) and electromyogram (EMG) signals that is compatible with commercial sleep-recording software that supports user datagram protocol (UDP) communication. The process consists of EEG/EMG data acquisition, automated threshold calculation for real-time determination of sleep stages, sleep staging and EEG power spectrum analysis. It also allows data storage in the format that facilitates further analysis of the sleep pattern in mice. The described method is aimed at increasing efficiency of sleep stage scoring and analysis in mice thus facilitating sleep research. • A process of EEG/EMG recording and streamline analysis of sleep-wake cycle in real time in mice. • The compatibility with commercial sleep-recording software that can generate a UDP stream. • The capability of further analysis of recorded data by an open-source software.
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