Stem/progenitor cells in normal physiology and disease of the pancreas.
Stem/progenitor cells in normal physiology and disease of the pancreas.
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DOI：
10.1016/j.mce.2021.111459
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发表时间：
2021-12-01
影响因子：
4.1
通讯作者：
Sancho R
中科院分区：
文献类型：
--
作者：
Alvarez Fallas ME;Pedraza-Arevalo S;Cujba AM;Manea T;Lambert C;Morrugares R;Sancho R
尽管胚胎胰腺祖细胞已被很好地表征，但在出生后哺乳动物胰腺中是否存在干细胞/祖细胞一直存在争议，主要原因是在小鼠损伤或疾病后再生的结果相互矛盾。尽管存在这些争议，但结合谱系追踪和类器官技术的测序进步表明，小鼠体内可能发生稳态和触发诱导的再生反应。在体内平衡和不同的应激挑战（如炎症、组织损伤和致癌应激）中，已经提出了成人胰腺中假定的祖细胞的存在。最近，单细胞转录组学揭示了所有胰腺细胞类型的显著异质性，其中一些细胞显示出潜在祖细胞的特征。在这篇综述中，我们概述了胚胎和假定的成年胰腺祖细胞在稳态和疾病中的作用，特别强调了体外培养系统和scRNA-seq技术作为解决不同胰腺细胞祖细胞性质的工具。据推测，胚胎期以后可能存在能够促进胰腺稳态的祖细胞。在胰腺的不同腔室中观察到异质性。胰腺肿瘤起源于不同的细胞，这决定了肿瘤的演变和预后。胰腺类器官培养为研究胚胎和成人祖细胞以及探索生成新β细胞的新方法提供了一个平台。单细胞转录组学允许预测成人和小鼠胰腺内的多能细胞群。
Though embryonic pancreas progenitors are well characterised, the existence of stem/progenitor cells in the postnatal mammalian pancreas has been long debated, mainly due to contradicting results on regeneration after injury or disease in mice. Despite these controversies, sequencing advancements combined with lineage tracing and organoid technologies indicate that homeostatic and trigger-induced regenerative responses in mice could occur. The presence of putative progenitor cells in the adult pancreas has been proposed during homeostasis and upon different stress challenges such as inflammation, tissue damage and oncogenic stress. More recently, single cell transcriptomics has revealed a remarkable heterogeneity in all pancreas cell types, with some cells showing the signature of potential progenitors. In this review we provide an overview on embryonic and putative adult pancreas progenitors in homeostasis and disease, with special emphasis on in vitro culture systems and scRNA-seq technology as tools to address the progenitor nature of different pancreatic cells. The existence of putative progenitors beyond the embryonic stage capable of contributing to pancreas homeostasis has been suggested. Heterogeneity is observed in the different compartments in the pancreas. Tumours in the pancreas arise from different originating cells that determine tumour evolution and prognosis. Pancreatic organoid cultures provide a platform to study embryonic and adult progenitors and to explore new ways of generating new β-cells. Single cell transcriptomics allowed the prediction of multipotent cell populations within the adult human and murine pancreas.
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影响因子：
9.3
作者：
Baron M;Veres A;Wolock SL;Faust AL;Gaujoux R;Vetere A;Ryu JH;Wagner BK;Shen-Orr SS;Klein AM;Melton DA;Yanai I
通讯作者：
Yanai I
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
16.6
作者：
Byrnes LE;Wong DM;Subramaniam M;Meyer NP;Gilchrist CL;Knox SM;Tward AD;Ye CJ;Sneddon JB
通讯作者：
Sneddon JB
影响因子：
10.9
作者：
Benninger, Richard K. P.;Piston, David W.
通讯作者：
Piston, David W.
影响因子：
4.7
作者：
Carvalho, Joao
通讯作者：
Carvalho, Joao