Lineage dynamics of murine pancreatic development at single-cell resolution.
Lineage dynamics of murine pancreatic development at single-cell resolution.
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DOI：
10.1038/s41467-018-06176-3
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发表时间：
2018-09-25
影响因子：
16.6
通讯作者：
Sneddon JB
中科院分区：
文献类型：
--
作者：
Byrnes LE;Wong DM;Subramaniam M;Meyer NP;Gilchrist CL;Knox SM;Tward AD;Ye CJ;Sneddon JB
器官发生需要多个细胞谱系的复杂相互作用，这些细胞谱系随着时间的推移协调它们的扩增、分化和成熟。在这里，我们配置文件的上皮和间充质隔室的小鼠胰腺在整个发展时间内的细胞类型，使用单细胞RNA测序，免疫荧光，原位杂交和遗传谱系追踪相结合。我们确定以前低估的细胞异质性的发展间充质和重建胰腺间皮细胞和间充质细胞类型之间的潜在谱系关系。在上皮内，我们发现了一个以前未描述的内分泌祖细胞群体，以及在人类胎儿组织和人类胚胎干细胞中分化为胰腺β细胞命运的类似群体。此外，我们确定候选转录调控沿着分化轨迹的这一人口向α或β细胞谱系。这项工作建立了胰腺发育的路线图，并证明了这种方法在了解器官发育中的谱系动力学方面的广泛实用性。不同细胞群的协调增殖和分化驱动胰腺上皮和间充质发育。在这里，作者使用单细胞RNA测序，鉴定间充质亚型和未描述的内分泌祖细胞，描述发育中小鼠胰腺的细胞类型动态。
Organogenesis requires the complex interactions of multiple cell lineages that coordinate their expansion, differentiation, and maturation over time. Here, we profile the cell types within the epithelial and mesenchymal compartments of the murine pancreas across developmental time using a combination of single-cell RNA sequencing, immunofluorescence, in situ hybridization, and genetic lineage tracing. We identify previously underappreciated cellular heterogeneity of the developing mesenchyme and reconstruct potential lineage relationships among the pancreatic mesothelium and mesenchymal cell types. Within the epithelium, we find a previously undescribed endocrine progenitor population, as well as an analogous population in both human fetal tissue and human embryonic stem cells differentiating toward a pancreatic beta cell fate. Further, we identify candidate transcriptional regulators along the differentiation trajectory of this population toward the alpha or beta cell lineages. This work establishes a roadmap of pancreatic development and demonstrates the broad utility of this approach for understanding lineage dynamics in developing organs. Coordinated proliferation and differentiation of diverse cell populations drive pancreatic epithelial and mesenchymal development. Here, the authors profile cell type dynamics in the developing mouse pancreas using single-cell RNA sequencing, identifying mesenchymal subtypes and undescribed endocrine progenitors.
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影响因子：
5.5
作者：
Hernandez-Torres F;Rodríguez-Outeiriño L;Franco D;Aranega AE
通讯作者：
Aranega AE
影响因子：
2.7
作者：
Heller, RS;Stoffers, DA;Serup, P
通讯作者：
Serup, P
DOI：
10.1161/01.atv.0000070546.16946.3a
发表时间：
2003-07-01
影响因子：
8.7
作者：
Frank, PG;Woodman, SE;Lisanti, MP
通讯作者：
Lisanti, MP
DOI：
10.1016/j.bbrc.2008.06.121
发表时间：
2008-09-12
影响因子：
3.1
作者：
Chiellini, Chiara;Grenningloh, Gabriele;Amri, Ez-Zoubir
通讯作者：
Amri, Ez-Zoubir
影响因子：
5.7
作者：
Huang C;Xiang Y;Chen S;Yu H;Wen Z;Ye T;Sun H;Kong H;Li D;Yu D;Chen B;Zhou M
通讯作者：
Zhou M