Sensitive absorbance detection method for capillary electrophoresis based on laser wave-mixing.

Sensitive absorbance detection method for capillary electrophoresis based on laser wave-mixing.
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基于激光混波的毛细管电泳灵敏吸光度检测方法

DOI:
10.1016/s0021-9673(97)00185-4
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发表时间:
1997
期刊:
Journal of chromatography. A
影响因子:
--
通讯作者:
Tong,WG
Tong,WG
中科院分区:
--
文献类型:
--
作者:
Wu,Z;Tong,WG

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前向散射四波混频被证明是一种灵敏的毛细管电泳吸光度检测方法,使用氩离子激光器在457.9 nm处工作。由于这种四波混合激光技术仅利用两个输入激光束,因此它提供了重要的优点,包括易于光学对准、高混合效率和低激发功率要求。另外,由于分析信号是激光类相干光束,因此可以以最小的光学背景噪声进行高效的光学信号检测。出色的检测灵敏度和短的吸收路径长度,因此,小检测器探针体积,是一些有用的功能,这种吸光度检测方法提供了荧光和非荧光分析物在毛细管电泳和液相色谱柱上检测。使用这种吸光度检测方法,确定了达比磺酰甘氨酸的初步“检测”浓度检测限为8.5·10−8M,质量检测限为13阿莫尔M,吸光度单位检测限为1.35·10−5AU。
Forward-scattering four-wave mixing is demonstrated as a sensitive absorbance detection method for capillary electrophoresis, using an argon ion laser operating at 457.9 nm. Since this four-wave mixing laser technique utilizes only two input laser beams, it offers important advantages, including ease of optical alignment, high wave-mixing efficiency and low excitation power requirements. In addition, since the analytical signal is a laser-like coherent beam, highly efficient optical signal detection can be performed with minimum optical background noise. Excellent detection sensitivity and short absorption path lengths, and hence, small detector probe volumes, are some of the useful features this absorbance detection method offers for on-column detection of both fluorescing and non-fluorescing analytes in capillary electrophoresis and liquid chromatography. Preliminary “detected” concentration detection limit of 8.5·10−8M, mass detection limit of 13 amol and an absorbance-unit detection limit of 1.35·10−5AU are determined for dabsyl-glycine using this absorbance detection method.
基于流动液体分析池中简并四波混合的光相位共轭的激光分析光谱测定。
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