Sensitive absorbance detection method for capillary electrophoresis based on laser wave-mixing.
Sensitive absorbance detection method for capillary electrophoresis based on laser wave-mixing.
复制标题
基于激光混波的毛细管电泳灵敏吸光度检测方法
DOI:
10.1016/s0021-9673(97)00185-4
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发表时间:
1997
期刊:
影响因子:
--
通讯作者:
Tong,WG
中科院分区:
文献类型:
--
作者:
Wu,Z;Tong,WG
Forward-scattering four-wave mixing is demonstrated as a sensitive absorbance detection method for capillary electrophoresis, using an argon ion laser operating at 457.9 nm. Since this four-wave mixing laser technique utilizes only two input laser beams, it offers important advantages, including ease of optical alignment, high wave-mixing efficiency and low excitation power requirements. In addition, since the analytical signal is a laser-like coherent beam, highly efficient optical signal detection can be performed with minimum optical background noise. Excellent detection sensitivity and short absorption path lengths, and hence, small detector probe volumes, are some of the useful features this absorbance detection method offers for on-column detection of both fluorescing and non-fluorescing analytes in capillary electrophoresis and liquid chromatography. Preliminary “detected” concentration detection limit of 8.5·10−8M, mass detection limit of 13 amol and an absorbance-unit detection limit of 1.35·10−5AU are determined for dabsyl-glycine using this absorbance detection method.
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影响因子:
7.4
作者:
Wu,ZQ;Tong,WG
通讯作者:
Tong,WG
DOI:
--
发表时间:
1991
期刊:
影响因子:
--
作者:
A. Bruno;A. Paulus;D. Bornhop
通讯作者:
D. Bornhop
DOI:
10.1016/0584-8547(92)80037-h
发表时间:
1992
期刊:
Spectrochimica Acta Part B: Atomic Spectroscopy
影响因子:
--
作者:
Zhiqiang. Wu;W. Tong
通讯作者:
W. Tong
影响因子:
3.4
作者:
Dennis A. Chen;W. Tong
通讯作者:
W. Tong
DOI:
--
发表时间:
1992
期刊:
影响因子:
--
作者:
K. Waldron;N. Dovichi
通讯作者:
N. Dovichi