Cancer stem-like cells enriched in Panc-1 spheres possess increased migration ability and resistance to gemcitabine.
Cancer stem-like cells enriched in Panc-1 spheres possess increased migration ability and resistance to gemcitabine.
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DOI：
10.3390/ijms12031595
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发表时间：
2011
影响因子：
5.6
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Yin T;Wei H;Gou S;Shi P;Yang Z;Zhao G;Wang C
胰腺癌是最致命的恶性肿瘤之一，预后不良。先前，我们发现Panc-1胰腺癌细胞系中的癌症干细胞（CSCs）亚群可以繁殖形成球体。在这里，我们描述了胰腺癌干细胞CD44+/CD24+细胞的恶性表型，通过流式细胞术分析，这些细胞在球形条件下富集。这些细胞对吉西他滨的耐药性增强，迁移能力增强。此外，这些细胞表现出上皮向间质转化的特征，其特征是上皮标志物e -钙粘蛋白水平降低，间质标志物vimentin水平升高。值得注意的是，Bmi-1、ABCG2、Cyclin D1和p16在Panc-1 CSCs中表达异常。我们的研究结果表明，靶向抑制CSCs是克服胰腺癌化疗耐药和转移的一种新的治疗方法。
Pancreatic cancer is one of the most lethal malignancies with poor prognosis. Previously, we found that a subpopulation of cancer stem cells (CSCs) in the Panc-1 pancreatic cancer cell line could propagate to form spheres. Here we characterized the malignant phenotypes of the pancreatic cancer stem CD44+/CD24+ cells, which were enriched under sphere forming conditions as analyzed by flow cytometry. These cells demonstrated increased resistance to gemcitabine and increased migration ability. Moreover, these cells exhibited epithelial to mesenchymal transition characterized by a decreased level of the epithelial marker E-cadherin and an increased level of the mesenchymal marker vimentin. Notably, abnormal expression of Bmi-1, ABCG2, Cyclin D1 and p16 were found in Panc-1 CSCs. Our results suggest that targeted inhibition of CSCs represents a novel therapeutic approach to overcome chemoresistance and metastasis of pancreatic cancer.
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