Differential Expression of PGC1α in Intratumor Redox Subpopulations of Breast Cancer.
Differential Expression of PGC1α in Intratumor Redox Subpopulations of Breast Cancer.
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DOI：
10.1007/978-3-319-91287-5_28
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发表时间：
2018
影响因子：
--
通讯作者：
Li LZ
中科院分区：
文献类型：
--
作者：
Lin Z;Xu HN;Wang Y;Floros J;Li LZ
Our previous studies indicate that the mitochondrial redox state and its intratumor heterogeneity are associated with invasiveness and metastatic potential in human breast cancer cell models and mouse xenografts. To further study the molecular basis of redox heterogeneity, we obtained the fluorescence images of Fp (oxidized flavoproteins containing flavin adenine dinucleotide, i.e., FAD), NADH (reduced nicotinamide adenine dinucleotide), and the Fp redox ratio (FpR = Fp/(Fp + NADH)) of MDA-MB-231 xenografts by the Chance redox scanner, then isolated the intratumoral redox subpopulations by dissection according to the redox ratio image. A total of 12 subpopulations were isolated from 4 tumors (2–4 locations from each tumor). The 12 subpopulations were classified into 3 FpR groups: high FpR (HFpR, n = 4, FpR range 0.78–0.92, average 0.85), medium FpR (MFpR, n = 5, FpR range 0.39–0.68, average 0.52), and low FpR (LFpR, n = 3, FpR range 0.15–0.28, average 0.20). The RT-PCR (reverse transcription polymerase chain reaction) analysis on these redox subpopulations showed that PGC-1α is significantly upregulated in the HFpR redox group compared to the MFpR group (fold change 2.1, p = 0.008), but not significantly different between MFpR and LFpR groups, or between HFpR and LFpR groups. These results indicate that optical redox imaging (ORI)-based redox subpopulations exhibit differential expression of PGC1α gene and suggest that PGC1α might play a role in redox mediation of breast cancer progression.
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影响因子：
11.2
作者：
Jorgenson TC;Zhong W;Oberley TD
通讯作者：
Oberley TD
DOI：
10.1073/pnas.0901807106
发表时间：
2009-04-21
影响因子：
11.1
作者：
Li, Lin Z.;Zhou, Rong;Glickson, Jerry D.
通讯作者：
Glickson, Jerry D.
影响因子：
28.2
作者：
Cantor JR;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1007/978-1-4614-4989-8_48
发表时间：
2013-01-01
期刊：
OXYGEN TRANSPORT TO TISSUE XXXIV
影响因子：
--
作者：
Xu, H. N.;Tchou, J.;Li, L. Z.
通讯作者：
Li, L. Z.
影响因子：
9
作者：
通讯作者：
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